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X-ray crystal structure of the light-independent protochlorophyllide reductase
TLDR
A unique trans-specific reduction mechanism is proposed in which the distorted C17-propionate of Pchlide and an aspartate from BchB serve as proton donors for C18 and C17 of PChlide, respectively. Expand
Protochlorophyllide reduction: a key step in the greening of plants.
  • Y. Fujita
  • Biology, Medicine
  • Plant & cell physiology
  • 1 June 1996
TLDR
The identification of genes for the reduction of Pchlide provides the groundwork for investigations of the mechanism that regulates the synthesis of Chl, which is closely coordinated with greening in plants. Expand
Chlorophyll biosynthesis: spotlight on protochlorophyllide reduction.
TLDR
This review discusses the two fundamentally different mechanisms of Pchlide reduction in photosynthetic organisms, Rhodobacter capsulatus and Rhodob bacteria. Expand
Regulation and evolution of chlorophyll metabolism.
  • T. Masuda, Y. Fujita
  • Biology, Medicine
  • Photochemical & photobiological sciences…
  • 17 September 2008
TLDR
The regulation and evolution of chlorophyll metabolism is discussed, based on recent findings, and identification of biosynthetic genes from various organisms and genetic analysis of functions of identified genes enables to predict the evolutionary scenario of chloropyll metabolism. Expand
Reconstitution of Light-independent Protochlorophyllide Reductase from Purified Bchl and BchN-BchB Subunits
TLDR
The first reproducible demonstration of dark protochlorophyllide reductase activity from purified protein subunits that were isolated from the purple nonsulfur photosynthetic bacterium Rhodobacter capsulatus is reported. Expand
Nitrate assimilatory genes and their transcriptional regulation in a unicellular red alga Cyanidioschyzon merolae: genetic evidence for nitrite reduction by a sulfite reductase-like enzyme.
TLDR
An overall scheme for nitrate assimilation in C. merolae is proposed, based on the complete genome sequence, after it was found that a CMG021G null mutant in comparison with the parental strain showed decreased cell growth in nitrate-containing but not in ammonium-containing media. Expand
Suppression of Calpain-dependent Cleavage of the CDK5 Activator p35 to p25 by Site-specific Phosphorylation*
TLDR
Results suggest phosphorylation of Thr138 predominantly defines the susceptibility of p35 to calpain-dependent cleavage and that dephosphorylated of this site is a critical determinant of Cdk5-p25-induced cell death associated with neurodegeneration. Expand
Calpain‐mediated degradation of p35 to p25 in postmortem human and rat brains
TLDR
It is shown that p35 is rapidly cleaved to p25 in rat and human brains within a short postmortem delay and that the conversion of p35 to p 25 is partially dependent on calpain activity. Expand
A Second Nitrogenase-like Enzyme for Bacteriochlorophyll Biosynthesis
TLDR
It is demonstrated that the B-ring of chlorin is reduced by a second nitrogenase-like enzyme and that the sequential actions of two nitrogen enzyme-like enzymes, DPOR and COR, convert porphyrin to bacteriochlorin. Expand
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