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Comparison of Gene Expression in Male and Female Mouse Blastocysts Revealed Imprinting of the X-Linked Gene, Rhox5/Pem, at Preimplantation Stages
Mammalian male preimplantation embryos develop more quickly than females . Using enhanced green fluorescent protein (EGFP)-tagged X chromosomes to identify the sex of the embryos, we compared geneExpand
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Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa
Formation of spermatozoa of normal shape, number, and motility is insufficient for the male siring of pups. The spermatozoa must be accompanied by sound fertilizing ability. We found that males withExpand
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Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA
CRISPR/Cas mediated genome editing has been successfully demonstrated in mammalian cells and further applications for generating mutant mice were reported by injecting humanized Cas9 (hCas) mRNA andExpand
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CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice
Targeted gene disrupted mice can be efficiently generated by expressing a single guide RNA (sgRNA)/CAS9 complex in the zygote. However, the limited success of complicated genome editing, such asExpand
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SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia
SPACA1 is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm-egg fusion. We produced a Spaca1 gene-disrupted mouse line andExpand
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Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive
Mouse work may lead to male contraceptive Unintended pregnancies are a major health issue worldwide. Although oral contraceptives were developed decades ago for use in women, there are no male oralExpand
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GPI-Anchored Protein Complex, LY6K/TEX101, Is Required for Sperm Migration into the Oviduct and Male Fertility in Mice1
ABSTRACT A disintegrin and metallopeptidase domain 3 (ADAM3) is a sperm membrane protein reported to be critical for both sperm migration from the uterus into the oviduct in vivo and sperm binding toExpand
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Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa
The identification, cloning, and gene disruption of two novel testis-specific proteins are reported. Pmis2-knockout males produce normal-looking spermatozoa but are sterile because of a severe defectExpand
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JunB is essential for IL-23-dependent pathogenicity of Th17 cells
CD4+ T-helper cells producing interleukin-17 (IL-17), known as T-helper 17 (TH17) cells, comprise heterogeneous subsets that exhibit distinct pathogenicity. Although pathogenic and non-pathogenicExpand
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Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naïve from primed pluripotent stem cells
Pluripotent stem cells can be classified into two distinct states, naïve and primed, which show different degrees of potency. One difficulty in stem cell research is the inability to distinguishExpand
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