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LEDGF/p75 Is Essential for Nuclear and Chromosomal Targeting of HIV-1 Integrase in Human Cells*
We have reported that human immunodeficiency virus type 1 (HIV-1) integrase (IN) forms a specific nuclear complex with human lens epithelium-derived growth factor/transcription co-activator p75Expand
HIV-1 Integrase Forms Stable Tetramers and Associates with LEDGF/p75 Protein in Human Cells*
The findings indicate that the minimal IN molecule in human cells is a homotetramer, suggesting that at least an octamer of IN is required to accomplish coordinated integration of both retroviral long terminal repeats and that LEDGF is a cellular factor involved in this process. Expand
The Endocrine Disruptor Monoethyl-hexyl-phthalate Is a Selective Peroxisome Proliferator-activated Receptor γ Modulator That Promotes Adipogenesis*
Structural and functional analyses are performed that demonstrate how monoethyl-hexyl-phthalate (MEHP) directly activates PPARγ and promotes adipogenesis, albeit to a lower extent than the full agonist rosiglitazone. Expand
Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequencyExpand
The Interaction of LEDGF/p75 with Integrase Is Lentivirus-specific and Promotes DNA Binding*
LEDGF/p75 interacted with HIV-1, HIV-2, and feline immunodeficiency virus IN, but not with the IN of human T-cell lymphotropic virus type 2, Moloney murine leukemia virus, or Rous sarcoma virus, and these results strongly suggest that the interaction of LEDGF/ p75 with IN is specific to lentiviridae. Expand
Dynamical and mechanical study of immobilized microtubules with atomic force microscopy
The dynamics of the assembly/disassembly reaction play an important role in the study of microtubule (MT) polymers. The polymerization rate constant (2±1×10−3 s−1) of this process was determined onExpand
Prolyl oligopeptidase stimulates the aggregation of α-synuclein
It is found that in vitro incubation of the protein with PO did not result in truncation of full-length alpha-synuclein, and an acceleration of the aggregation process of alpha- synuclein using turbidity measurements that was reversed by specific inhibitors of PO enzymatic activity. Expand
The Protein Phosphatase 2A Phosphatase Activator Is a Novel Peptidyl-Prolyl cis/trans-Isomerase*
This study demonstrates that PTPA and its yeast homologues Ypa1 and Ypa2 can induce a conformational change in some model substrates and identifies Pro190 in the catalytic subunit of PP2A (PP2AC) as the target Pro isomerized by PTPA/Mg2+ATP. Expand
Biliverdin reductase is a transporter of haem into the nucleus and is essential for regulation of HO-1 gene expression by haematin.
Experiments with nuclear localization, export signal mutants and si-hBVR [siRNA (small interfering RNA) specific to hBVR] indicate that nuclear localization of hB VR is required for induction of HO-1 by haematin. Expand
Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes.
Data show that wild-type penetratin and the two analogues interact with negatively charged phospholipids, and that this is accompanied by a conformational change from random to alpha helical structure, and a deeper insertion of W48 compared to W56, into the lipid bilayer. Expand