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Beta-amyrin synthase--cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants.
The present studies have revealed that distinct OSC exists for triterpene formation in higher plants, and the high level of similarity with cycloartenol synthase indicates close evolutional relationship between sterol and triterPene biosynthesis. Expand
Identification of β‐amyrin and sophoradiol 24‐hydroxylase by expressed sequence tag mining and functional expression assay
Successful identification of a β‐amyrin and sophoradiol 24‐hydroxylase from the inducible family of cytochrome P450 genes suggests that other triterpene hydroxylases belong to this family, and substrate specificity with the obtained P450 hydroxyase indicates the two possible biosynthetic routes from triterPene‐monool to triter pene‐triol. Expand
Identification of Claisen cyclase domain in fungal polyketide synthase WA, a naphthopyrone synthase of Aspergillus nidulans.
Since the C-terminus region of WA PKS of this type was determined to be involved in Claisen-type cyclization of the second ring of naphthopyrone, it is proposed that the so far called TE of these PKSs work not just as TE but as Claisen cyclase. Expand
Solanapyrone Synthase, a Possible Diels–Alderase and Iterative Type I Polyketide Synthase Encoded in a Biosynthetic Gene Cluster from Alternaria solani
The purified recombinant SPS showed activity for the formation of (−)‐solanapyrone A from achiral prosolanAPyrone II, establishing that this single enzyme catalyzes both the oxidation and the subsequent cycloaddition reaction, possibly as a Diels–Alder enzyme. Expand
Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-Methylsalicylic acid synthase gene by heterologous expression
Abstract Southern blot analysis of genomic DNAs of several fungi that produce polyketide compounds with the 6-methylsalicylic acid synthase (MSAS) gene of Penicillium patulum as a probe indicated theExpand
A new pathway for polyketide synthesis in microorganisms
It is shown that a homodimer of RppA catalyses polyketide synthesis: it selects malonyl-coenzyme-A as the starter, carries out four successive extensions and releases the resulting pentaketide to cyclize to 1,3,6,8-tetrahydroxynaphthalene (THN). Expand
p-Coumaroyltriacetic acid synthase, a new homologue of chalcone synthase, from Hydrangea macrophylla var. thunbergii.
Two cDNA clones obtained from leaves of Hydrangea macrophylla var. Expand
Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max
Results suggest that soyasaponin I is biosynthesized from soyasapogenol B by successive sugar transfer reactions, and glycosyltransferases from Glycine max are identified and characterized. Expand
Molecular cloning and functional expression of triterpene synthases from pea (Pisum sativum) new alpha-amyrin-producing enzyme is a multifunctional triterpene synthase.
Ursane type triterpene is one of the most widespread triterpene aglycones found in plants, together with oleanane type, and these two types often occur together in the same plant. Pisum sativum isExpand
Re-identification of Aspergillus nidulans wA gene to code for a polyketide synthase of naphthopyrone
Abstract By reconstruction and expression of the full-length WA (NWA) polyketide synthase of A. nidulans based on the revised wA gene sequence, its product was re-identified to be a naphthopyroneExpand