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Beta-amyrin synthase--cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants.
The present studies have revealed that distinct OSC exists for triterpene formation in higher plants, and the high level of similarity with cycloartenol synthase indicates close evolutional relationship between sterol and triterPene biosynthesis.
Identification of β‐amyrin and sophoradiol 24‐hydroxylase by expressed sequence tag mining and functional expression assay
- M. Shibuya, M. Hoshino, Y. Katsube, H. Hayashi, T. Kushiro, Y. Ebizuka
- Biology, ChemistryThe FEBS journal
- 1 March 2006
Successful identification of a β‐amyrin and sophoradiol 24‐hydroxylase from the inducible family of cytochrome P450 genes suggests that other triterpene hydroxylases belong to this family, and substrate specificity with the obtained P450 hydroxyase indicates the two possible biosynthetic routes from triterPene‐monool to triter pene‐triol.
Identification of Claisen cyclase domain in fungal polyketide synthase WA, a naphthopyrone synthase of Aspergillus nidulans.
Solanapyrone Synthase, a Possible Diels–Alderase and Iterative Type I Polyketide Synthase Encoded in a Biosynthetic Gene Cluster from Alternaria solani
- Ken Kasahara , T. Miyamoto, I. Fujii
- Biology, ChemistryChembiochem : a European journal of chemical…
- 14 June 2010
The purified recombinant SPS showed activity for the formation of (−)‐solanapyrone A from achiral prosolanAPyrone II, establishing that this single enzyme catalyzes both the oxidation and the subsequent cycloaddition reaction, possibly as a Diels–Alder enzyme.
Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max
[Biosynthesis of plant pigments].
Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-Methylsalicylic acid synthase gene by heterologous expression
- I. Fujii, Yuya Ono, H. Tada, K. Gomi, Y. Ebizuka, U. Sankawa
- BiologyMolecular and General Genetics MGG
- 27 November 1996
Abstract Southern blot analysis of genomic DNAs of several fungi that produce polyketide compounds with the 6-methylsalicylic acid synthase (MSAS) gene of Penicillium patulum as a probe indicated the…
A new pathway for polyketide synthesis in microorganisms
- N. Funa, Y. Ohnishi, I. Fujii, M. Shibuya, Y. Ebizuka, S. Horinouchi
- Chemistry, BiologyNature
- 26 August 1999
It is shown that a homodimer of RppA catalyses polyketide synthesis: it selects malonyl-coenzyme-A as the starter, carries out four successive extensions and releases the resulting pentaketide to cyclize to 1,3,6,8-tetrahydroxynaphthalene (THN).
p-Coumaroyltriacetic acid synthase, a new homologue of chalcone synthase, from Hydrangea macrophylla var. thunbergii.
- T. Akiyama, M. Shibuya, H. M. Liu, Y. Ebizuka
- Chemistry, BiologyEuropean journal of biochemistry
- 1 August 1999
Two cDNA clones obtained from leaves of Hydrangea macrophylla var.