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Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters
Abstract High molecular weight polyhydroxyalkanoates (PHA) are synthesized and stored in the cell cytoplasm as water-insoluble inclusions by various microorganisms. This intriguing biologicalExpand
High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain
Abstract High yield production of polyhydroxyalkanoates (PHAs) by Ralstonia eutropha H16 and its recombinant strain PHB−4/pJRDEE32d13 (a PHA-negative mutant harboring Aeromonas caviae PHA synthaseExpand
Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation
Large-scale fermentative production of poly(3-hydroxybutyrate-co-5mol% 3-hydroxyhexanoate) [P(3HB-co-5mol% 3HHx)] from soybean oil as sole carbon source is simulated using a recombinant strain ofExpand
Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
Aeromonas caviae produced a random copolymer of 3-hydroxybutyric acid (3HB) and 3-hydroxyhexanoic acid (3HH) under aerobic conditions when sodium salts of alkanoic acids of even carbon numbersExpand
Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae
The cloned fragments could not only complement PHA-negative mutants of Alcaligenes eutrophus and Pseudomonas putida, but also confer the ability to synthesize P(3HB-co-3HHx) from octanoate or hexanoate on the mutants' hosts. Expand
Expression and Characterization of (R)-Specific Enoyl Coenzyme A Hydratase Involved in Polyhydroxyalkanoate Biosynthesis by Aeromonas caviae
Results have demonstrated that (R-specific hydration of 2-enoyl-CoA catalyzed by the translated product of phaJ(Ac) is a channeling pathway for supplying (R)-3-hydroxyacyl- coenzyme A monomer units from fatty acid beta-oxidation to poly(3-Hydroxybutyrate-co-3- hydroxyhexanoate) biosynthesis in A. caviae. Expand
Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans.
  • Y. Saito, Y. Doi
  • Materials Science, Medicine
  • International journal of biological…
  • 1 March 1996
The biodegradabilities of P(3HB-co-4HB) films were studied in aqueous solutions of extracellular polyhydroxybutyrate (PHB) depolymerase from Alcaligenes faecalis or of lipase from Rhizopus delemer, and the erosion rate of films by lipase increased with the 4HB fraction. Expand
Thermal degradation of poly((R)-3-hydroxybutyrate), poly(e-caprolactone), and poly((S)-lactide)
Thermal degradations of poly[(R)-3-hydroxybutyrate] (PHB), poly[e-caprolactone] (PCL), and poly[(S)-lactide] (PLA) were investigated under both isothermal and non-isothermal conditions. In isothermalExpand
Efficient production of polyhydroxyalkanoates from plant oils by Alcaligenes eutrophus and its recombinant strain
  • T. Fukui, Y. Doi
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • 1 March 1998
It is demonstrated that renewable and inexpensive plant oils are excellent carbon sources for efficient production of PHA using A. eutrophus strains from olive oil. Expand