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Polyamine synthesis in plants: isolation and characterization of spermidine synthase from soybean (Glycine max) axes.
The enzyme activity is inhibited by N-ethylmaleimide and DTNB, but stimulated by Co2+, Cu2+ and Ca2+ significantly, suggesting that these metal ions could be the cellular regulators in polyamine biosynthesis. Expand
Identification of essential active-site residues in ornithine decarboxylase of Nicotiana glutinosa decarboxylating both L-ornithine and L-lysine.
The three-dimensional model of ODC protein constructed on the basis of the crystal structure of Trypanosoma brucei, mouse and human ODCs localized the four residues in the active- site cleft, the first work carried out on active-site residues of plant ODC, where ODC and LDC activities occur in the same catalytic site. Expand
Purification and Properties of Arginase from Soybean, Glycine max, Axes.
Arginase (EC 3.1) was purified to homogeneity from cytosol of soybean, Glycine max, axes by chromatographic separations on Sephadex G-200, DEAE-sephacel, hydroxyapatite, and arginine-affinity columns and stimulated by polyamines such as putrescine. Expand
Purification and properties of transglutaminase from soybean (Glycine max) leaves.
With molecular weight of this enzyme, this inhibition of enzyme activity by GTP indicates that this enzyme is very similar to known tissue transglutaminases in animals. Expand
Isolation and properties of arginase from a shade plant, ginseng (Panax ginseng C.A. Meyer) roots.
Specific activities of the enzyme in sliced ginseng roots were increased by plant hormones such as GA(3), IAA, kinetin and putrescine, whereas the Activities of the purified enzyme were unaffected by putres cine, suggesting increases in arginase activities by these plant hormones could affect metabolism of polyamine intracellularly. Expand
Purification and characterization of monomeric lysine decarboxylase from soybean (Glycine max) axes.
Lysine decarboxylase (EC 4.1.18) was purified 364-fold from 2-day-old soybean (Glycine max) axes and was competitively inhibited by Cl- with a Ki value of 1.46 mM, however, the activity of the purified enzyme was not inhibited by F-, Br, I, H2PO4, HPO4, or SO4(2-). Expand
Resolution and unambiguous identification of microgram amounts of 22 lupin alkaloids by sequential use of thin-layer and gas-liquid chromatography and mass spectrometry.
Mass spectrometry has been successfully applied to the free base forms of 20 individual quinolizidine alkaloids collected from GLC, making possible the complete resolution of all these compounds. Expand
Identification of functionally important residues of Arabidopsis thaliana S-adenosylmethionine decarboxylase.
Results suggest that the lysine(81) residue is critical for substrate binding in the AdoMetDC protein, demonstrating that lysyl and sulfhydryl groups are required for full catalytic activity. Expand
Uptake of [3H]glycine and [14C]glutamate by cultures of chick spinal cord
1. Spinal cord explants from chick embryos, grown in culture for up to 16 days, rapidly accumulated [3H]glycine and [14C]glutamate when incubated at 25° C in a medium containing either 2 × 10−10 M… Expand
Kinetic properties of the GABA uptake system in cultures of chick retina.