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A multidisciplinary approach for molecular diagnostics based on biosensors and microarrays
- J. Cloarec, Y. Chevolot, Emmanuelle Laurenceau, M. Phaner-Goutorbe, É. Souteyrand
- 1 April 2008
Abstract The present review describes the multidisciplinary approach followed by the Chemistry and Nanobiotechnology group of INL since ten years, to develop a complete technological platform… Expand
DNA-based carbohydrate biochips: a platform for surface glyco-engineering.
MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms
- A. Groisillier, C. Hervé, +12 authors M. Czjzek
- Biology, Medicine
- Microbial cell factories
- 14 June 2010
BackgroundThe production of stable and soluble proteins is one of the most important steps prior to structural and functional studies of biological importance. We investigated the parallel production… Expand
Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads
- N. Jaffrezic-Renault, C. Martelet, Y. Chevolot, J. Cloarec
- Sensors (Basel, Switzerland)
- 1 April 2007
This review paper reports the applications of magnetic microbeads in biosensors and bio-bar code assays. Affinity biosensors are presented through different types of transducing systems:… Expand
Surface modification of PVC endotracheal tubes by oxygen glow discharge to reduce bacterial adhesion
A d.c. oxygen glow discharge was used to modify medical-grade poly(vinyl chloride) (PVC) to study how surface chemistry and hydrophilicity influence Pseudomonas aeruginosa adhesion. The effects of… Expand
Application of microfluidic chip with integrated optics for electrophoretic separations of proteins.
- Julien Vieillard, R. Mazurczyk, +4 authors S. Krawczyk
- Chemistry, Medicine
- Journal of chromatography. B, Analytical…
- 15 January 2007
This paper describes the fabrication, the characterization and the applications of a capillary electrophoresis microchip. This hybrid device (glass/PDMS) features channels and optical waveguides… Expand
Multiplexed binding determination of seven glycoconjugates for Pseudomonas aeruginosa lectin I (PA-IL) using a DNA-based carbohydrate microarray.
The binding of seven multivalent glycoconjugates displaying linear or antenna-like structures and different electronic environments were evaluated towards PA-IL on a DNA-based carbohydrate… Expand
Carbodiimide/NHS derivatization of COOH-terminated SAMs: activation or byproduct formation?
- F. Palazon, Cindy Montenegro Benavides, D. Léonard, É. Souteyrand, Y. Chevolot, Jean-Pierre Cloarec
- Chemistry, Medicine
- Langmuir : the ACS journal of surfaces and…
- 16 April 2014
COOH-terminated self-assembled monolayers (SAMs) are widely used in biosensor technology to bind different amine-containing biomolecules. A covalent amide bond, however, can be achieved only if the… Expand
Silanization of silica and glass slides for DNA microarrays by impregnation and gas phase protocols: A comparative study
Abstract Surface immobilization of oligonucleotide probes (oligoprobes) is a key issue in the development of DNA-chips. The immobilization protocol should guarantee good availability of the probes,… Expand
Toward the Rational Design of Galactosylated Glycoclusters That Target Pseudomonas aeruginosa Lectin A (LecA): Influence of Linker Arms That Lead to Low-Nanomolar Multivalent Ligands.
Anti-infectious strategies against pathogen infections can be achieved through antiadhesive strategies by using multivalent ligands of bacterial virulence factors. LecA and LecB are lectins of… Expand