• Publications
  • Influence
The sigma-1 receptor protects against cellular oxidative stress and activates antioxidant response elements.
TLDR
Significant changes in protein levels were identified by two-dimensional gel electrophoresis and mass spectrometry and sigma-1 receptor mediated activation of the antioxidant response element (ARE) to upregulate NAD(P)H quinone oxidoreductase 1 (NQO1) and superoxide dismut enzyme 1 (SOD1) mRNA expression in COS cells was shown by RT PCR. Expand
Biophysical Characterization of the Interaction Domains and Mapping of the Contact Residues in the XPF-ERCC1 Complex*
TLDR
The cross-saturation data and the crystal structure of related proteins to model the two complexes revealed that XPF interacts with ERCC1 mainly through hydrophobic interactions, and XPF-EB was also shown to homodimerize in the absence of ER CC1. Expand
Lipid Binding Ridge on Loops 2 and 3 of the C2A Domain of Synaptotagmin I as Revealed by NMR Spectroscopy*
TLDR
The results show that the “docking ridge” responsible for Ca2+-dependent membrane association is localized on the opposite side of the C2A domain from the transmembrane and C2B domains of synaptotagmin. Expand
Structure-function relationships in Anabaena ferredoxin: correlations between X-ray crystal structures, reduction potentials, and rate constants of electron transfer to ferredoxin:NADP+ reductase for
TLDR
It is concluded that nonconservative mutations of three critical residues (S47, F65, and E94) on the surface of ferredoxin have large parallel effects on both the reduction potential and the electron-transfer reactivity of the [2Fe-2S] cluster, thereby making electron transfer thermodynamically feasible. Expand
Structure-function studies of [2Fe-2S] ferredoxins
TLDR
Results have shown that serine can replace cysteine at each of the four cluster attachment sites and still support cluster assembly, and electron transfer has been demonstrated with three of theFour vegetative ferredoxin mutants. Expand
NMR solution structure of ATTp, an Arabidopsis thaliana trypsin inhibitor.
TLDR
The three-dimensional structure of the precursor form of the Arabidopsis thaliana trypsin inhibitor (ATTp, GenBank entry Z46816), a 68-residue rapeseed class proteinase inhibitor, has been determined by multinuclear magnetic resonance spectroscopy. Expand
Solution structure of the toluene 4-monooxygenase effector protein (T4moD).
TLDR
The specificity of T4moD as an effector protein was investigated by replacing it in reconstituted T4MO complexes with effector proteins from monooxygenases from other bacterial species by refined through simulated annealing by molecular dynamics in torsion angle space. Expand
Acquisition of heat shock tolerance by regulation of intracellular redox states.
TLDR
This study confirms that Hsp104p plays a crucial role in keeping cells from being damaged by the oxidative stress, thus acting as a modulator of the intracellular redox state. Expand
NMR solution structure of ATTp, an Arabidopsis thaliana trypsin inhibitor.
TLDR
The overall fold of ATT(p) is distinct from those of other classes of serine proteinase inhibitors except in the inhibitor loop; therefore, it represents a new inhibitor fold. Expand
Amino acid residues in Anabaena ferredoxin crucial to interaction with ferredoxin-NADP+ reductase: site-directed mutagenesis and laser flash photolysis.
TLDR
Spectroscopic and thermodynamic measurements with both the E94 and F65 mutants indicated that the kinetic differences cannot be ascribed to changes in gross conformation, redox potential, or FNR binding constant but rather reflect the protein-protein interactions that control electron transfer. Expand
...
1
2
3
4
5
...