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MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis
MicrobeJ, a plug-in for the open-source platform ImageJ1, provides a comprehensive framework to process images derived from a wide variety of microscopy experiments with special emphasis on large image sets, and provides a robust way to verify the accuracy and veracity of the data.
The structure of FtsZ filaments in vivo suggests a force‐generating role in cell division
In prokaryotes, FtsZ (the filamentous temperature sensitive protein Z) is a nearly ubiquitous GTPase that localizes in a ring at the leading edge of constricting plasma membranes during cell…
In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria.
Cell cycle-dependent transcriptional and proteolytic regulation of FtsZ in Caulobacter.
It is proposed that CtrA is a repressor of ftsZ transcription, and proteolysis is an important determinant of cell type-specific distribution and cell cycle variation of FtsZ.
Getting in the Loop: Regulation of Development in Caulobacter crescentus
A portion of many developmental processes of Caulobacter crescentus is reviewed, with particular emphasis on how multiple processes are integrated and coordinated both spatially and temporally.
MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae
It is shown that both phosphorylated and non-phosphorylated forms of MapZ are required for proper Z-ring formation and dynamics and illustrates that nature has evolved a diversity of cell division mechanisms adapted to the different bacterial clades.
A new metabolic cell wall labeling method reveals peptidoglycan in Chlamydia trachomatis
This work used a novel approach to metabolically label chlamydial PG using d-amino acid dipeptide probes and click chemistry and found that the results of differential probe incorporation experiments conducted in the presence of ampicillin are consistent with the existence of chlamYDial PG-modifying enzymes.
The Caulobacter crescentus polar organelle development protein PodJ is differentially localized and is required for polar targeting of the PleC development regulator
The results indicate that PodJ is an important determinant for the localization of a major regulator of cell differentiation, and a novel role for this protein in controlling the dynamic localization of the developmental regulator PleC is described.
Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division
To understand how these components coordinate to divide cells, visualized their movements relative to the dynamics of cell wall synthesis during cytokinesis and found that the division septum was built at discrete sites that moved around the division plane.
Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ
A modular synthesis protocol is provided for the synthesis of four FDAAs emitting light in blue, green, green and fluorescein-D-lysine or red for their use in PG labeling of live bacteria and several scenarios for the use of these labels in fluorescence microscopy applications are discussed.