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Diet-Dependent Shifts in the Bacterial Population of the Rumen Revealed with Real-Time PCR
The real-time PCR technique uncovered differential amplification of rumen bacterial templates with the set of universal bacterial primers, which may explain why some predominant rumen bacteria have not been detected in PCR-generated 16S ribosomal DNA libraries. Expand
Rumen Bacterial Community Transition During Adaptation to High-grain Diet
Transitional changes of the ruminal bacterial community structure in cows during the switch from roughage to high-grain diet were monitored by PCR amplification and sequencing of 16S rDNA cloneExpand
Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage.
Two selected strains, Lactobacillus casei and L. plantarum, improved fermentation quality but did not inhibit the growth of silage yeast or aerobic deterioration of the silage. Expand
Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries
The bacterial community structure was revealed by phylogenetic placement of sequences in relation to different fractions of rumen content and the vast majority of sequences from the rumen solids were found to be related to low G+C Gram-positive bacteria and the remaining sequences were placed within the Cytophaga-Flexibacter-Bacteroides and Spirochaetes phyla. Expand
Innate lymphoid cells regulate intestinal epithelial cell glycosylation
It is shown that ILC3-mediated epithelial fucosylation protects the host from invasion of S. typhimurium into the intestine, and the critical role of the cytokines IL-22– and/or LT-producing I LC3 in the induction and regulation of intestinal epithelium fucOSylation is demonstrated. Expand
Impact of Intestinal Microbiota on Intestinal Luminal Metabolome
Analysis of the colonic luminal metabolome using capillary electrophoresis mass spectrometry with time-of-flight with CE-TOFMS indicates that intestinal microbiota highly influenced the Colonic luminals metabolome and a comprehensive understanding of intestinal luminal metabolites is critical for clarifying host-intestinal bacterial interactions. Expand
Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella.
Nine strains capable of metabolizing isoflavones to equol were isolated from human faeces and a new genus is proposed, Adlercreutzia equolifaciens sp. Expand
Phylogenetic Analysis of the Human Gut Microbiota Using 16S rDNA Clone Libraries and Strictly Anaerobic Culture‐Based Methods
The human gut microbiota from three healthy subjects were compared by the use of a sequence analysis of 16S rDNA libraries and a culture‐based method and showed marked individual differences in the composition of intestinal microbiota. Expand
Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.
A new genus, Tannerella, is proposed for Bacteroides forsythus, with one species, TANNerella forsythensis corrig. Expand
DNA relatedness among Erysipelothrix rhusiopathiae strains representing all twenty-three serovars and Erysipelothrix tonsillarum.
The results confirm that the genus Erysipelothrix contains two main genomic species, E. rhusiopathiae and E. tonsillarum, which can be differentiated into serovars. Expand