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Kinetic and equilibrium properties are compared for a monomeric kinesin construct (K332) and a dimeric construct (K379). MtK379 has a low affinity (5 x 10(4) M(-1)) and a high affinity (5 x 10(6) M(-1)) binding site for mant ADP while MtK332 has a single low affinity site (5 x 10(4) M(-1)). Rate constants of dissociation of mant ADP are <1 s(-1) for the(More)
The kinetic mechanism of myofibril ATPase was investigated using psoas and mixed back muscle over a range of ionic strengths. Myofibrils were labeled with pyrene iodoacetamide to measure the rate constants for the binding of ATP and formation of the weakly attached state. The velocity of shortening was measured by stopping the contraction at various times(More)
We have studied the effect of the absence of carotenoids on the organization of bacteriochlorophylls (BChls) in chlorosomes of Chlorobium (Chl.) phaeobacteroides strain CL1401. Carotenoid-depleted chlorosomes were obtained by means of 2-hydroxybiphenyl-supplemented cultures. In the presence of the inhibitor, isorenieratene (Isr) and beta-Isr biosynthesis(More)
The kinetic mechanism is analyzed for a monomeric human kinesin construct K332. In the absence of microtubules, the rate constants of the ATPase cycle are very similar to dimeric human kinesin K379 and whole kinesin from bovine brain. The microtubule-activated ATPase is 60 s(-1) at 20 degrees C; Km(Mt) is 5 microM; dissociation constants in the presence of(More)
A six-step mechanism is derived for the activation of kinesin K379 ATPase by microtubules. The data are fitted by the kinetic scheme [Formula see text] where T, D, and P refer to nucleotide triphosphate, nucleotide diphosphate, and inorganic phosphate, respectively; MtK refers to the complex of a K379 unit with the microtubule binding site. The initial(More)
The kinetic mechanism of the human kinesin ATPase motor domain K379, expressed in Escherichia coli, was determined by transient and steady-state kinetic studies. The steps in nucleotide binding were measured using the fluorescent substrate analogues, methylanthraniloyl ATP (mant-ATP) and mant-ADP. Both nucleotides gave a two-step fluorescence signal, an(More)
REPLICA is a special-purpose computer architecture to support active multi-sensory perception of 3-D objects. The system is partitionable, reconfigurable, and highly parallel. In this paper, we present the design of a set of interconnection networks which form the core of the REPLICA architecture. This set of interconnection networks consists of a(More)
Characterization of rheological behavior of alkali-surfactant-polymer (ASP) solution and oil emulsion is difficult, due to the complex chemical components and various physiochemical reactions with oil during chemical flooding. Through rheological experiments of ASP and crude oil emulsion, this paper presents the studies on influencing factors of rheological(More)
We discuss petrophysical analysis of wireline logs, methods for formation evaluation and issues in evaluating tight sandstone formations. The main concerns of log interpretation in tight gas sandstones include porosity interpretation, understanding the effect of clay on the log responses, accurate computation of water saturation, and permeability(More)