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Calcium has an important role in the events of egg activation and early preimplantation development. We investigated changes in intracellular calcium concentration in human oocytes at fertilization using the calcium-sensitive photoprotein aequorin. Oocytes were donated for research by patients undergoing in-vitro fertilization treatment in the Department of(More)
The mechanisms of calcium signalling in mammalian oocytes during maturation and fertilization are controversial. In this study we measured intracellular free Ca2+ concentrations ([Ca2+]i) with the photoprotein aequorin microinjected into immature mouse oocytes. Immature mouse oocytes typically produced [Ca2+]i responses to muscarinic acetylcholine (ACh)(More)
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