Learn More
Single crystal aluminum and copper of (001) and (110) orientation were shock peened using laser beam of 12 micron diameter and observed with X-ray micro-diffraction techniques based on a synchrotron light source. The X-ray micro-diffraction affords micron level resolution as compared with conventional X-ray diffraction which has only mm level resolution.(More)
The immunofluorescence-based detection of γ-H2AX is a reliable and sensitive method for quantitatively measuring DNA double-strand breaks (DSBs) in irradiated samples. Since H2AX phosphorylation is highly linear with radiation dose, this well-established biomarker is in current use in radiation biodosimetry. At the Center for High-Throughput Minimally(More)
In this paper, we describe a novel strategy for combining fisher's linear discriminant (FLD) preprocessing with a feedforward neural network to classify cultured cells in bright field images. This technique was applied to various experimental scenarios utilizing different imaging environments, and the results were compared with those for the traditional(More)
A completely automated, high-throughput biodosimetry workstation has been developed by the Center for Minimally Invasive Radiation Biodosimetry at Columbia University over the past few years. To process patients' blood samples safely and reliably presents a significant challenge in the development of this biodosimetry tool. In this paper, automated failure(More)
The immunofluorescence-based detection of c-H2AX is a reliable and sensitive method for quantitatively measuring DNA double-strand breaks (DSBs) in irradiated samples. Since H2AX phosphorylation is highly linear with radiation dose, this well-established biomarker is in current use in radiation biodosimetry. At the Center for High-Throughput Minimally(More)
Design aspects of a minimally invasive high-throughput automation system for radiation biodosimetry are reported. The system, currently under development, relies on robotic devices and advanced high-speed automated image acquisition to perform mass triage following a radiological event. A design concept of the automation system is proposed based on the use(More)
Electron backscatter diffraction (EBSD) is used to investigate crystal lattice rotation caused by plastic deformation during high-strain rate laser shock peening in single crystal aluminum and copper sample on ͑11 ¯ 0͒ and (001) surfaces. New experimental meth-odologies are employed which enable measurement of the in-plane lattice rotation under approximate(More)
Laser shock peening (LSP) is a process to improve material fatigue life by introducing compressive residual surface stress in a target. The residual stresses are introduced when a high-intensity laser impinges on an ablative layer deposited on the surface of the target material. The interaction between laser and the ablative layer creates a high pressure(More)