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BACKGROUND AND AIMS The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of intermediate oily seeds using Citrus as a model. METHODS The relationships between equilibrium relative humidity (RH), seed water content, presence of freezable water as determined by DSC analysis, and germination percentage(More)
This paper investigates the importance of loading and treatment with a vitrification solution on the survival of Citrus madurensis embryonic axes cryopreserved using a vitrification protocol. Among the seven different loading solutions tested, the solution containing 2 M glycerol + 0.4 M sucrose was the most efficient. Of the six vitrification solutions(More)
The role of pregrowth and preculture treatments in terms of both medium composition and exposure duration on survival of embryonic axes of Citrus madurensis after cryopreservation using the vitrification procedure was investigated. The optimal pregrowth treatment for excised embryonic axes was a 3-day treatment with 0.1M sucrose. Preculture was also(More)
  • CHENG YEW KUANG, Dr Bernard Thong, Y L Hor, Cheng Yew Kuang
  • 2006
There is an increasing worldwide and regional prevalence of allergic rhinitis and asthma. Aeroallergens are important triggers in allergic rhinitis and asthma although other genetic and environmental factors also play a role in ongoing airway inflammation. Up to 20-30% of patients with allergic rhinitis have asthma. Up to 60% of patients with asthma have(More)
In this paper, we demonstrate that C. madurensis embryonic axes can withstand cryopreservation using the encapsulation-dehydration technique. Up to 57.5 % survival was achieved using a standard encapsulation-dehydration protocol, which included pregrowth of encapsulated axes for 16 h in medium containing 0.8 M sucrose + 1 M glycerol, desiccation of beads to(More)
Adverse reactions to food (food hypersensitivity) may either be immunological (food allergy) or non-immunological (nonallergic food hypersensitivity or intolerance). Undiagnosed food allergy may result in fatal anaphylaxis, especially in children and adolescents with asthma. Skin prick tests, measurement of allergen-specific IgE levels and food challenges(More)
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