Learn More
Effects of nicotine on circadian rhythms of ambulatory activity and drinking in male Wistar rats were examined. Nicotine was administered through the drinking water, and the daily doses of nicotine were adjusted to 0.5, 5 and 20 mg/kg/day. The treatment of nicotine induced a dose-dependent increase in ambulatory activity. On the other hand, fluid intake(More)
Circadian variation of nicotine-induced ambulatory activity in drug-naive rats was investigated. To test ambulatory activity, male Wistar rats (4 weeks of age) housed under a 12 hr light-dark cycle (light on from 6:00 to 18:00) with dawn and dusk periods (each over 2 hr), for 25 days, were injected with nicotine (0.1 or 0.5 mg/kg) at one of six times each(More)
Nicotinic acetylcholine receptor (nAChR) purified from rat brains by cholinergic ligand affinity chromatography was characterized. Monoclonal antibody 299, which binds an acetylcholine (ACh) binding subunit termed alpha 4, depleted more than 85% of [3H]ACh binding activity of the purified preparation. A number of cholinergic agonists strongly inhibited(More)
Nicotine (0.5 and 1.0 mg/kg) administered subcutaneously to mice decreased the ambulatory activity recorded by an ambulo-meter in a dose-dependent manner from 5 to 60 min after the administration, and the higher dose (1.0 mg/kg) caused a long-lasting ataxia. To be noted was the initial increment of ambulation which usually preceded the ataxia-inducing(More)
The effects of staurosporine and K-252a, potent inhibitors of protein kinases, and 12-O-tetradecanoylphorbol-13-acetate (TPA) on catecholamine secretion and protein phosphorylation in digitonin-permeabilized bovine adrenal medullary cells were investigated. Staurosporine and K-252a (0.01-10 microM) did not cause large changes in catecholamine secretion(More)
A convenient and sensitive method for the determination of 15NH3 has been developed. Ammonia was purified from sample solutions by a modified microdiffusion method, derivatized with pentafluorobenzoyl chloride to pentafluorobenzamide (PFBA) and determined by gas chromatography-mass spectrometry using a multiple ion detector. PFBA was eluted from the gas(More)