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Circadian variation of nicotine-induced ambulatory activity in drug-naive rats was investigated. To test ambulatory activity, male Wistar rats (4 weeks of age) housed under a 12 hr light-dark cycle (light on from 6:00 to 18:00) with dawn and dusk periods (each over 2 hr), for 25 days, were injected with nicotine (0.1 or 0.5 mg/kg) at one of six times each(More)
Nicotinic acetylcholine receptor (nAChR) purified from rat brains by cholinergic ligand affinity chromatography was characterized. Monoclonal antibody 299, which binds an acetylcholine (ACh) binding subunit termed alpha 4, depleted more than 85% of [3H]ACh binding activity of the purified preparation. A number of cholinergic agonists strongly inhibited(More)
Using chromatography on DE-52 and acetylcholine-Affi-Gel columns, nicotinic acetylcholine receptor was purified to approximately 10,000 fold from Lubrol extract of rat brain with a recovery of 15%. The purified preparation contained no cholinesterase activity. alpha-Bungarotoxin did not inhibit [3H]acetylcholine binding to the purified preparation. Sodium(More)
Male Wistar rats, aged 4 weeks, were kept in a temperature controlled room with a 12 hr light-dark cycle and given food and water ad libitum. The nicotine-treated group of rats was given water containing nicotine, which was estimated to be 10 mg/kg/day, for 40 days. Spontaneous motor activities, drinking activities and serum corticosterone levels showed(More)
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