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Qualitative and quantitative alterations of G proteins in membrane preparations from parietal and temporal cortex regions in post-mortem brains obtained from alcoholics and controls matched with respect to age and post-mortem delay were investigated by Western-blotting with polyclonal antibodies against specific G protein subunits and functional(More)
The present study examined the in vitro effects of antidepressants on functional photoaffinity labeling of GTP binding protein. Saturation binding studies were performed by incubating membranes with increasing concentrations of [32P]-AAGTP, followed by UV irradiation and SDS-PAGE. The specifically bound isotherms for each of the G proteins studied showed(More)
Lymphocyte adenylate cyclase (AC) activity in alcoholics was examined. Gpp(NH)p-stimulated AC activity was not altered in lymphocyte membranes from alcoholics in acute withdrawal state. In the case of long-term abstinent alcoholics, who maintained abstinence from alcohol for at least one year, lymphocyte Gpp(NH)p-stimulated AC activity was slightly reduced(More)
Platelet GTP binding protein (G protein) in 25 alcoholics with a first-degree alcoholic relative (family history positive alcoholic; FHP), in 25 alcoholics without alcoholic relatives (family history negative alcoholic; FHN) and in 25 controls without alcoholic relatives was investigated to determine whether this protein is a trait marker for alcoholism.(More)
The effect of ethanol "in vitro" on GTP-binding protein in postmortem human brain cortex membranes was analyzed using by photoaffinity hydrolysis resistant GTP analog (azidoanilido GTP, AAGTP). AAGTP identified stimulatory GTP binding protein [Gs alpha (42 KDa)] and inhibitory GTP binding protein [Gi alpha (40 KDa)] in human brain cortex membranes. Ethanol(More)
This study investigated alterations in the receptor adenylyl cyclase system in the brain and platelets of alcoholics through the study of GTP binding (G) protein, which has a key role in the system, in the membranes of the post-mortem brain and platelets. Quantitative examination of G protein by immunoblotting showed that GsH alpha in the temporal cortex of(More)
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