Y I Zilberter

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Fast inactivation of Na channels in neonatal cardiac cells was removed by the action of proteolytic enzymes trypsin or papain. Two stages were apparent in the time course of this process. During the first one, both number of channel reopenings and the mean open time increased markedly even though fast inactivation remained complete. The second stage was(More)
Block of cardiac sodium channels is enhanced by repetitive depolarization. It is not clear whether the changes in drug binding result from a change in affinity that is dependent on voltage or on the actual state of the channel. This question was examined in rabbit ventricular myocytes by analyzing the kinetics of block of single sodium channel currents with(More)
In voltage-clamp studies of atrial myocytes exposed to disopyramide or quinidine, pulse-train stimulation revealed use-dependent block that increased with increased pulse amplitude. Use-dependent block also became negligible at hyperpolarized holding potentials (< -150 mV), consistent with either rapid unbinding at the holding potential or trapping of the(More)
A patch-voltage-clamp method was used to measure fast inward ionic currents in single heart muscle cells. 1. Theoretical analysis including computer simulation has shown that the method provides fast settling of membrane potential (within 10 microseconds) and reliable voltage clamp on the tested membrane patch when the area of the patch is 200-300 times(More)
"Fast chemical stimulation" was shown to induce potentiation of glutamate-activated currents in neurons isolated from rat hippocampus. A fast application system allowed solution changes up to a rate of 20 Hz. In Mg2(+)-free solution, the response to glutamate application immediately after repetitive stimulation with glutamate plus glycine was increased by(More)
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