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We have investigated hierarchical capped channels that provide 3D microtubule confinement (Huang et al., 2004). We describe here a simplified polymethymethacrylate bonded (PMMA) 3D hierarchical channel process. This process provides shallow motor microchannels (4-6 mum wide and 1.5 mum deep) and deeper structures (~250 mum deep) for fluid input and output(More)
A continuing challenge in nanoscience is the manipulation and assembly of materials at the nanoscale. Kinesin-microtubule biomotors [1] provide a model system for biologically derived nanoscale motion. By using a combination of kinesin functionalization and geometric confinement by channel walls [2], directional movement of microtubules moving over kinesin(More)
Kinesins are molecular motors that move along microtubules, and provide a model system for force generation that can be exploited for kinesin-powered nano- and micro-machines. Microtubules are /spl sim/25 nm diameter cylindrical polymers of the protein tubulin and can be nm to /spl mu/m long. Kinesins bind to microtubules and use the energy of ATP(More)
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