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Protease inhibitors against trypsin were extracted from cowpea seeds, purified, and characterized. After the seed powder was defatted with hexane, the cowpea trypsin inhibitor (CpTI) was extracted with 0.15 M NaCl for 30 min. The crude extracts were then heated at 90°C for 10 min, followed by precipitation with 40-65% saturation ammonium sulfate, by which(More)
Life-cycle assessment (LCA) was used to evaluate the energy efficiency and environmental impacts of sweet potato-based bioethanol production. The scope covered all stages in the life cycle of bioethanol production, including the cultivation and treatment, transport, as well as bioethanol conversion of sweet potato. Results show that the net energy ratio of(More)
Life cycle analysis method was used to evaluate the energy efficiency and environmental performance of bioethanol production from sweet sorghum stem in China. The scope covers three units, including plant cultivation, feedstock transport, and bioethanol conversion. Results show that the net energy ratio was 1.56 and the net energy gain was 8.37 MJ/L. Human(More)
China's industry accounts for 46.8% of the national Gross Domestic Product (GDP) and plays an important strategic role in its economic growth. On the other hand, industrial wastewater is also the major source of water pollution. In order to examine the relationship between the underlying driving forces and various environmental indicators, values of two(More)
Life cycle assessment was conducted to evaluate the energy efficiency and environmental impacts of a bioethanol production system that uses sweet sorghum stem on saline-alkali land as feedstock. The system comprises a plant cultivation unit, a feedstock transport unit, and a bioethanol conversion unit, with 1000L of bioethanol as a functional unit. The net(More)
Anaerobic co-digestion (A co-D) performance of Phragmites australis, feces and kitchen waste with addition of clinoptilolite (one main kind of zeolite) was investigated to evaluate the improvement of biogas/methane production and internal mechanism of nitrogen and organics control. A better biogas/methane production was observed by 10% clinoptilolite (v/v)(More)
Marbofloxacin is a fluoroquinolone veterinary antibiotic. An indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of marbofloxacin using polyclonal antibody. The half-maximum inhibition concentrations (IC(50)) and limit of detection (LOD, calculated as IC(20)) of the ELISA for marbofloxacin in phosphate buffer were(More)
In this study, the removal of volatile solids (VSs) and soluble chemical oxygen demand (SCOD) by co-digesting cattle dung (CD) and duck manure (DM) was determined and compared with the reduction achieved with CD or DM digestion alone. Moreover, fluorescence excitation-emission matrix spectroscopy was utilised to characterise the conversion mechanisms of(More)
In order to strengthen the management of municipal plastics waste, the amount, development trend and factors interactions of the generation should be studied. On the basis of studying the dynamic response relationship between the generation course and the ciety, economy and environment, this paper established a system dynamics model about the generation(More)