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Sulfated, carboxylic polysaccharides and polyphenols found in marine macro-algae interfere with RNA isolation from these plants and inhibit RNA activities in vitro. Methods based on differential precipitation of RNA or carbohydrates in high salts were used to eliminate the acidic carbohydrates. To protect RNA from inactivation by oxidized polyphenols,(More)
Some BK channels are activated in response to membrane stretch. However, it remains largely unknown which membrane component transmits forces to the channel and which part of the channel senses the force. Recently, we have shown that a BK channel cloned from chick heart (named SAKCa channel) is a stretch activated channel, while deletion of a 59 amino acids(More)
RNA accumulation in Chlamydomonas reinhardtii during the course of wall regeneration in protoplasts prepared by treatment of vegetative cells with autolysin was studied. In vitro translation of poly(A)+ RNA isolated from wall-regenerating cells indicated that cell wall removal induced the accumulation of several large RNA transcripts. Some of these induced(More)
  • X Su
  • Analytical biochemistry
  • 1987
A refined silver staining method was developed to stain nucleic acids fixed onto nitrocellulose membranes and nylon-based membranes. Approximately 4 ng RNA or DNA can be stained with this method with no protein interference. This method involves simple repetition of immersions of membranes in three solutions prepared from common chemicals. The total(More)
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