• Publications
  • Influence
Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
Derivatives of Escherichia coli C were engineered to produce primarily succinate or malate in mineral salts media using simple fermentations (anaerobic stirred batch with pH control) without theExpand
Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli
Results of deleting individual transport genes confirmed that GalP served as the dominant glucose transporter in evolved strains and increased the pool of PEP available for redox balance by eliminating the need to produce additional PEP from pyruvate. Expand
Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C
Derivatives of Escherichia coli C were previously described for succinate production by combining the deletion of genes that disrupt fermentation pathways for alternative products with growth‐based selection for increased ATP production to create a strain devoid of foreign DNA. Expand
Fermentation of Glycerol to Succinate by Metabolically Engineered Strains of Escherichia coli
The fermentative metabolism of Escherichia coli was reengineered to efficiently convert glycerol to succinate under anaerobic conditions without the use of foreign genes to solve problems of insufficient energy production or insufficient levels of electron acceptors. Expand
Engineering central metabolic modules of Escherichia coli for improving β-carotene production.
Combined engineering of TCA and PPP modules had a synergistic effect on improving β-carotene yield, leading to 64% increase of β-vehicle yield over a high producing parental strain. Expand
Producing aglycons of ginsenosides in bakers' yeast
The yeast strains engineered in this work can serve as the basis for creating an alternative way for producing ginsenosides in place of extractions from plant sources and provides an alternative route compared to traditional extraction methods. Expand
Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization
Combinatorial modulation of galactose permease (galP) and glucokinase (glk) gene expression was carried out in chromosome of an Escherichia coli strain with inactivated PTS to improve anaerobic glucose utilization rate for efficient production of succinate and malate. Expand
Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides.
The yeast strains engineered in this work can serve as the basis for creating an alternative way for production of ginsenosides in place of extraction from plant sources. Expand
Production of l-alanine by metabolically engineered Escherichia coli
In these strains, reduced nicotinamide adenine dinucleotide oxidation during alanine biosynthesis is obligately linked to adenosine triphosphate production and cell growth, which provided a basis for metabolic evolution where selection for improvements in growth coselected for increased glycolytic flux andAlanine production. Expand
Activating transhydrogenase and NAD kinase in combination for improving isobutanol production.
It was found that increasing pntAB gene expression alone had a threshold for improving anaerobic isobutanol production, while activating NAD kinase could break through this threshold, leading to a yield of 0.92mol/mol. Expand