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Pentachlorophenol (PCP) is a highly toxic contaminant of chlorophenols. Due to its slow and incomplete biodegradation, it can be found in surface, groundwater and in soils. To investigate the role of intracellular calcium and reactive oxygen species in apoptosis induced by PCP in cultured hepatocytes, the primary hepatocytes of Carassius carassius were(More)
Nitrate at 70 m gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4 +/NO3 − molar ratio of 5:1 (giving a total of 40 m N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280%(More)
The effects of eight microelements (I−, BO3 3−, MoO4 2−, Co2+, Cu2+, Mn2+, Fe2+, Zn2+) on the biosynthesis of camptothecin and the growth of suspension cultures of Camptotheca acuminata were studied. The increase of I− to 25 μM l−1, Cu2+ to 1 μM l−1, Co2+ to 2 μM l−1 and MoO4 2− to 10 μM l−1 in Murashige and Skoog (MS) medium resulted in 1.66, 2.84, 2.53(More)
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