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Structural features of human initiation factor 4E, studied by X-ray crystal analyses and molecular dynamics simulations.
SDS-PAGE analyses showed that this structural instability is highly related to the fast degradation of cap-free eIF4E, compared with cap-bound or 4E-BP/cap-bound eIF5E, indicating the conferment of structural stability of eIF 4E by the binary or ternary complex formation. Expand
Binding interaction of quercetin-3-β-galactoside and its synthetic derivatives with SARS-CoV 3CLpro: Structure–activity relationship studies reveal salient pharmacophore features
A new class of compounds are revealed as potential drug leads against the SARS virus, and a solid understanding of the mechanism of inhibition against the target enzyme is provided. Expand
Molecular Basis of the Interaction of Saccharomyces cerevisiae Eaf3 Chromo Domain with Methylated H3K36*
The molecular mechanism of the recognition and binding of the methylated H3K36 by Eaf3 is revealed and new insights are provided into the functional roles of the Eaf2 chromo domain. Expand
Carboxyl terminus of severe acute respiratory syndrome coronavirus nucleocapsid protein: self-association analysis and nucleic acid binding characterization.
Gel shift assay results revealed that the SARS-CoV N protein C-terminus is also able to associate with nucleic acids and residues 363-382 are the responsible interaction partner, demonstrating that this fragment might involve genomic RNA binding sites. Expand
Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E:
The present results provide the structural basis for the biological function of both N- and C-terminal mobile regions of eIF4E in translation initiation, especially the regulatory function through the switch-on/off of eif4E-binding protein-eIF 4E phosphorylation. Expand
Structural basis for mRNA Cap-Binding regulation of eukaryotic initiation factor 4E by 4E-binding protein, studied by spectroscopic, X-ray crystal structural, and molecular dynamics simulation
The dynamic regulation of the cap-binding of eIF4E by 4E-BP1 was investigated by molecular dynamics simulations, and the simulation suggested that the helical region of the 4E -BP1 peptide is important for the binding with eif4E. Expand
D-Alanine:D-alanine ligase as a new target for the flavonoids quercetin and apigenin.
It is determined that d-alanine:d-alanin ligase (Ddl) is another new target for quercetin and apigenin, and this work is expected to help shed more light on the potential antibacterial mechanism of flavonoids. Expand
Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori
A new aroE gene encoding SDH from Helicobacter’s strain SS1 is identified and expected to favor better understanding the features of SDH and provide useful information for the development of novel antibiotics to treat H.’pylori‐associated infection. Expand
Residues Asp164 and Glu165 at the substrate entryway function potently in substrate orientation of alanine racemase from E. coli: Enzymatic characterization with crystal structure analysis
  • Dalei Wu, T. Hu, +5 authors Xu Shen
  • Biology, Medicine
  • Protein science : a publication of the Protein…
  • 1 June 2008
It is proposed that the negatively charged residues Asp164 and Glu165 around the substrate entryway play an important role in substrate orientation with cooperation of the positively charged Arg280 and Arg300 on the opposite monomer. Expand
Cinanserin Is an Inhibitor of the 3C-Like Proteinase of Severe Acute Respiratory Syndrome Coronavirus and Strongly Reduces Virus Replication In Vitro
  • Lili Chen, C. Gui, +17 authors Hualiang Jiang
  • Biology, Medicine
  • Journal of Virology
  • 1 June 2005
It is demonstrated that the old drug cinanserin is an inhibitor of SARS-CoV replication, acting most likely via inhibition of the 3CL proteinase. Expand