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Four new terpenoids, metaseglyptorin A (1), metasequoic acid C (2), 12α-hydroxy-8,15-isopimaradien-18-oic acid (3), and (-)-acora-2,4(14),8-trien-15-oic acid (4), and three new norlignans, metasequirins D-F (5-7), were isolated from Metasequoia glyptostroboides, together with 15 known compounds. Structures of the new compounds were determined by analysis of(More)
The yeast Saccharomyces cerevisiae IMA multigene family encodes four isomaltases sharing high sequence identity from 65% to 99%. Here, we explore their functional diversity, with exhaustive in-vitro characterization of their enzymological and biochemical properties. The four isoenzymes exhibited a preference for the α-(1,6) disaccharides isomaltose and(More)
The effects of the interaction between Suillus luteus (L.) Roussel and Trichoderma virens (J.H. Mill., Giddens & A.A. Foster) Arx on Pinus sylvestris var. mongolica Litv. were studied using plant physiology, mycorrhizal science, forest pathology, and biochemistry. Seedling growth and physiological parameters were determined, including the colonization rate(More)
The skin-resident dendritic cells (DCs) are thought to be the first defender to encounter incoming viruses and likely play a role in Japanese encephalitis virus (JEV) early infection. In the current study, following the demonstration of JEV productive infection in DCs, we revealed that the interaction between JEV envelope glycoprotein (E glycoprotein) and(More)
The marine bacteria strains S1, S2 and S3, were isolated on seawater culturing medium containing Hg(2+), Cd(2+), Cr(6+) or Ni(2+) at concentrations of 20 mg L(-1) and more. The isolates showed tolerance to these heavy metals. S1 grew in the presence of 120 mg L(-1) of Hg(2+) and accumulated Hg(2+) at pH 4-10. For the effect of co-existing cations on(More)
A recombinant photosynthetic bacterium, Rhodopseudomonas palustris, was constructed to simultaneously express mercury transport system and metallothionein for Hg(2+) removal from heavy metal wastewater. The effects of essential process parameters, including pH, ionic strength and presence of co-ions on Hg(2+) uptake were evaluated. The results showed that(More)
An unprecedented copper-catalyzed intramolecular radical cyclization was developed for the synthesis of 3-hydroxypyrroloindoline skeletons in excellent yields. The 3-hydroxyl group was introduced by trapping the radical intermediate with molecular oxygen or TEMPO. This process represents a unique radical oxidation pathway for tryptamine/tryptophan(More)
The article extended the study on the bioaccumulation of cadmium by genetically engineered bacterium Escherichia coli (namely M4) simultaneously expressing a cadmium transport system and metallothionein (MT). The growth of M4 showed resistance to the presence of cadmium. Compared with Cd2+ uptake capacity by original host bacterial cells, The Cd2+(More)
This study constructed a genetically engineered Escherichia coli JM109 which simultaneously expressed nickel transport system and metallothionein to remove and recover Ni(2+) from aqueous solution. Bioaccumulation process was rapid and followed linearized Langmuir isotherm. A more than six-fold increase of Ni(2+) binding capacity was obtained by genetically(More)