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BACKGROUND MicroRNA (miRNA) is a class of small RNAs of ~22nt which play essential roles in many crucial biological processes and numerous human diseases at post-transcriptional level of gene expression. It has been revealed that miRNA genes tend to be clustered, and the miRNAs organized into one cluster are usually transcribed coordinately. This implies a(More)
OBJECTIVE To investigate oxidative DNA damage in pharmacy technicians preparing antineoplastic drugs at the PIVAS (Pharmacy Intravenous Admixture Service) in two Chinese hospitals. METHODS Urinary 8-OHdG served as a biomarker. 5-Fluorouracil (5-FU) concentrations in air, masks and gloves were determined. The spill exposure of each PIVAS technician to(More)
BACKGROUND The mitochondrial voltage-dependent anion channel (VDAC) is increasingly implicated in the control of apoptosis. We have studied the effects the mitochondrial DNA (mtDNA) tRNAIle mutation on VDAC expression, localization, and apoptosis. METHODS Lymphoblastoid cell lines were derived from 3 symptomatic and 1 asymptomatic members of a family with(More)
SUMMARY The great variety of human cell types in morphology and function is due to the diverse gene expression profiles that are governed by the distinctive regulatory networks in different cell types. It is still a challenging task to explain how the regulatory networks achieve the diversity of different cell types. Here, we report on our studies of the(More)
BACKGROUND Current approaches for identifying transcriptional regulatory elements are mainly via the combination of two properties, the evolutionary conservation and the overrepresentation of functional elements in the promoters of co-regulated genes. Despite the development of many motif detection algorithms, the discovery of conserved motifs in a wide(More)
Transcription factors (TFs) and miRNAs are essential for the regulation of gene expression; however, the global view of human gene regulatory networks remains poorly understood. For example, how is the expression of so many genes regulated by limited cohorts of regulators and how are genes differentially expressed in different tissues despite the genetic(More)
The tissue sample of lung cancer is imaged by phase contrast CT method based on synchrotron radiation in vitro, and the imaging results are reconstructed in three-dimensional limit. The experiments were performed at X-ray imaging and biomedical application beam-line (BL13W1) of Shanghai Synchrotron Radiation Facility (SSRF). The results show that lung(More)
Transcription factors (TFs) are important regulatory proteins that govern transcriptional regulation. Today, it is known that in higher organisms different TFs have to cooperate rather than acting individually in order to control complex genetic programs. The identification of these interactions is an important challenge for understanding the molecular(More)
In this paper, the x-ray diffraction enhanced imaging (DEI) of lung cancer was analyzed and the contrast of image was studied at the variant points of the rocking curve. Firstly, at the bottom of the rocking curve, the contrast of the image is highest but the clarity of the image is not good. Then, at the full width of half maximum of rocking curve, the(More)
MOTIVATION Identification of microRNA (miRNA) transcriptional start sites (TSSs) is crucial to understand the transcriptional regulation of miRNA. As miRNA expression is highly cell specific, an automatic and systematic method that could identify miRNA TSSs accurately and cell specifically is in urgent requirement. RESULTS A workflow to identify the TSSs(More)