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Mitochondria are subcellular organelles with an essentially oxidative type of metabolism. The production of reactive oxygen species (ROS) in it increases under stress conditions and causes oxidative damage. In the present study, effects of exogenous sodium nitroprusside (SNP), a nitric oxide (NO) donor, on both the ROS metabolism in mitochondria and(More)
The tuberous root of radish is an important vegetable, but insufficient transcriptomic and genomic data are currently available to understand the molecular mechanisms underlying tuberous root formation and development. High-throughput transcriptomic sequencing is essential to generate a large transcript sequence data set for gene discovery and molecular(More)
The study showed that in solar greenhouse continuously cropped cucumber soil, phenolic acids p-hydroxybenzoic acid, ferulic acid and benzoic acid had an obvious accumulation with increasing cropping year, and their contents were significantly higher after continuously cropped for 5 approximately 9 years than for 1 approximately 3 years. With the increasing(More)
Both H(2)O(2) and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallothionein in tomato plant responses to(More)
S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overexpressing SlSAMS1 exhibited a significant(More)
Nitric oxide (NO) and auxin (indole-3-acetic acid; IAA) play vital roles in regulating plants tolerance to abiotic stresses. This study showed that both NO and IAA could induce cucumber plants tolerance to sodic alkaline stress, which depended on their roles in regulating reactive oxygen species (ROS) scavenging, antioxidative enzymes activities, Na(+)(More)
Melatonin (MT) plays integral roles in regulating several biological processes including plant growth, seed germination, flowering, senescence, and stress responses. This study investigated the effects of MT on adventitious root formation (ARF) of de-rooted tomato seedlings. Exogenous MT positively or negatively influenced ARF, which was dependent on the(More)
The objective of this study was to develop an efficient regeneration and transformation protocol for cucumber (Cucumis sativus L.). Cotyledonary node explants showed a very high regeneration frequency (94.3 %) when incubated on Murashige and Skoog medium (MS) supplemented with 1.5 mg l−1 6-benzyladenine (BA). An Agrobacterium-mediated transformation(More)
The paper presents Greedy Randomized Adaptive Search Procedure with Path Relinking (GRASP with PR) for the Critical Node Detection Problem (CNDP). An evolutionary Path Relinking mechanism is added to GRASP with PR to intensify. Our computational experiments show that this algorithm is a competitive method compared with the previously proposed methods for(More)
In this research, biological function of CsNMAPK, encoding a mitogen-activated protein kinase of cucumber, was investigated under salt and osmotic stresses. Northern blot analysis showed that the expression of CsNMAPK was induced by salt and osmotic stresses in the cucumber root. In order to determine whether CsNMAPK was involved in plant tolerance to salt(More)