Xiufang Tang

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OBJECTIVES To develop a reliable HPLC method for the simultaneous determination of plasma tryptophan, kynurenine and 5-hydroxytryptamine to analyze tryptophan metabolism. DESIGN AND METHODS Separation was carried out on a C8 column with the mobile phase composed of acetate buffer (pH 4.5) and acetonitrile using theophylline as internal standard. The(More)
A precolumn derivatization-high performance liquid chromatographic method for the determination of homocysteine (Hcy) in plasma was established. Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) and N-(1-pyrenyl) maleimide (NPM) were used as the reduced reagent and derivatization reagent, respectively. The separation was carried out on an Agilent(More)
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