Xingjiang Li

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Environmental conditions, particularly pH, have significant effects on the efficiency and final titers of bio-based products. Therefore, these factors need to be identified to ensure the fermentation process is economically attractive. In this study, strategies for controlling pH were optimized to enhance succinic acid production by Corynebacterium crenatum(More)
Electrospinning technique was used to fabricate chloroamphenicol loaded poly (L-lactic acid)/ZrO2 (CP-PLLA/ZrO2) nanofibrous membranes. The average diameter of drug carrier PLLA/ZrO2 nanofibers increased with ZrO2 concentration increasing and ribbon-shape fibers were present when ZrO2 content reached 3% and above. The existence of hydrogen and ZrOC bonds(More)
OBJECTIVE In order to obtain high yield mutant strains for the industrial bioconversion of succinic acid, we analyzed the metabolic networks of the strain Actinobacillus succinogenes S.JST in the course of screening and breeding. METHODS We previously identified the wild-type strain by API biochemical reactions and 16S r RNA sequence analysis. Following(More)
It is very important to obtain high yield mutant strains on the base of metabolic flux analysis of Actinobacillus succinogenes S.JST for the industrial bioconversion of succinic acid. The metabolic pathway was analized at first and the flux of the metabolic networks was calculated by matrix. In order to decrease acetic acid flux, the strains mutated by soft(More)
To construct a system of genetic transformation suitable for Rhizopus oryzae, we constructed a single-exchange vector pBS-hygro carrying hygromycin B resistance gene (hph) as its selective marker using gene splicing by overlap extension PCR (SOE PCR) technique. We introduced this recombinant vector into Rhizopus oryzae AS 3.819 by PEG/CaCl2-mediated(More)
l-Lactic acid production by Rhizopus oryzae in a semi-continuous fermentation system has been recognized as a promising technology. We studied l-lactic acid production in ten batches of semi-continuous fermentation by R. oryzae using a 7-L stirred fermentor and found that the kinetics of the system was in good agreement with the logistic and Luedeking–Piret(More)
The replacement of the carbon source in the microbial production of itaconic acid (IA) with economic alternatives has attracted significant attention. In this study, an Aspergillus terreus CICC40205 mutant was used to increase the IA titer and decrease the citric acid titer in the wheat bran hydrolysate compared with the parental strain. The results showed(More)
A new succinic acid and lactic acid production bioprocess by Corynebacterium crenatum was investigated in mineral medium under anaerobic conditions. Corynebacterium crenatum cells with sustained acid production ability and high acid volumetric productivity harvested from the glutamic acid fermentation broth were used to produce succinic acid and lactic(More)
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