Xingjiang Li

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Acetobacter pasteurianus JST-S was screened from solid fermented grains of vinegar in China, identified by molecular analysis, and used for the production of purple sweet potato vinegar using purple sweet potato as the substrate. By orthogonal experiment, maximum total acid concentration (4.26% [v/v]) was achieved under optimized conditions as follows:(More)
With the growth of the world population, energy problems are becoming increasingly severe; therefore, sustainable energy sources have gained enormous importance. With respect to ethanol fuel production, biomass is gradually replacing grain as the main raw material. In this study, we explored the fermentation of five- and six-carbon sugars, the main biomass(More)
To construct a system of genetic transformation suitable for Rhizopus oryzae, we constructed a single-exchange vector pBS-hygro carrying hygromycin B resistance gene (hph) as its selective marker using gene splicing by overlap extension PCR (SOE PCR) technique. We introduced this recombinant vector into Rhizopus oryzae AS 3.819 by PEG/CaCl2-mediated(More)
l-Lactic acid production by Rhizopus oryzae in a semi-continuous fermentation system has been recognized as a promising technology. We studied l-lactic acid production in ten batches of semi-continuous fermentation by R. oryzae using a 7-L stirred fermentor and found that the kinetics of the system was in good agreement with the logistic and Luedeking–Piret(More)
Environmental conditions, particularly pH, have significant effects on the efficiency and final titers of bio-based products. Therefore, these factors need to be identified to ensure the fermentation process is economically attractive. In this study, strategies for controlling pH were optimized to enhance succinic acid production by Corynebacterium crenatum(More)
OBJECTIVE In order to obtain high yield mutant strains for the industrial bioconversion of succinic acid, we analyzed the metabolic networks of the strain Actinobacillus succinogenes S.JST in the course of screening and breeding. METHODS We previously identified the wild-type strain by API biochemical reactions and 16S r RNA sequence analysis. Following(More)
Electrospinning technique was used to fabricate chloroamphenicol loaded poly (L-lactic acid)/ZrO2 (CP-PLLA/ZrO2) nanofibrous membranes. The average diameter of drug carrier PLLA/ZrO2 nanofibers increased with ZrO2 concentration increasing and ribbon-shape fibers were present when ZrO2 content reached 3% and above. The existence of hydrogen and ZrOC bonds(More)
Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria (AAB). To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the(More)
The replacement of the carbon source in the microbial production of itaconic acid (IA) with economic alternatives has attracted significant attention. In this study, an Aspergillus terreus CICC40205 mutant was used to increase the IA titer and decrease the citric acid titer in the wheat bran hydrolysate compared with the parental strain. The results showed(More)