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Orthogonal optimization of prokaryotic expression of a natural snake venom phospholipase A2 inhibitor from Sinonatrix annularis.
A His6-PLIγ pET28 fusion expression vector and transformed Escherichia coli BL21 showed a good inhibitory effect of enzymatic activities to venom PLA2 isolated from D. acutus, Naja atra and Agkistrodon halys venom and had a wide anti-hemorrhage activities. Expand