Xing-yuan Jia

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To construct a safer and more efficient gene engineering Lactococcus Lactis for expressing phenylalaine ammonia lyase (PAL) which will be benefit for PKU therapy, pal cDNA of Parsly and synthesized sequence based on Lactococcus Lactis bias codons were recombined into two Lactococcus Lactis NICE systems. The activities of the expressed PAL were detected, and(More)
To establish a multiplex real-time fluorescence relative quantitative PCR method for diagnosis of Down's syndrome. The fragment from Down's syndrome critical region gene 3 (DSCR3) on chromosome 21 was used as the target gene, and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene on chromosome 12 was used as the control gene. The two genes were(More)
OBJECTIVE To establish a comprehensive and simple assay using denaturing high performance liquid chromatography (DHPLC) for the diagnosis of most common mutations and deletions of α-thalassemia gene in Southeast Asians and Southern Chinese. METHODS This assay has included a duplex polymerase chain reaction (PCR) followed by DHPLC analysis. An improved PCR(More)
OBJECTIVE To investigate the general anesthetic's effect upon cholinergic system to examine whether the regional and progressive cholinergic changes may lead to postoperative cognitive dysfunction (POCD). METHOD A model of hippocampus microdialysis was established in aging rats (18 months old). The tissue levels of choline (Ch) and acetylcholine (Ach)(More)
To construct a new high effective genetic engineering strain which can express active PAL enzyme in Lactococcus lactis (L.L), and acquire better effect on curing hyperphenylalaninemia rats, Firstly translational fusion vector and transcriptional fusion vector were constructed in E. coli MC1061, and then PAL cDNA was transformed into L.L. Two kinds of high(More)
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