Xing Zhen Chen

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Most studies on kidney development have considered the interaction of the metanephric mesenchyme and the ureteric bud to be the major inductive event that maintains tubular differentiation and branching morphogenesis. The mesenchyme produces Gdnf, which stimulates branching, and the ureteric bud stimulates continued growth of the mesenchyme and(More)
Polycystin-2 (PC2) is the product of the second cloned gene (PKD2) responsible for autosomal dominant polycystic kidney disease and has recently been shown to be a calcium-permeable cation channel. PC2 has been shown to connect indirectly with the actin microfilament. Here, we report a direct association between PC2 and the actin microfilament. Using a(More)
The wild type proA+, B+ genes of E. coli were cloned in vivo using a plasmid containing a mini-Mu replicon, pEG5005. The cloning frequency was about 1.46 x 10(-3)/Kanr transductant. Genetic and biochemical analysis of these clones indicated that the proA+, B+ genes are on the plasmid pEG5005. The secretion of proline were assayed for 500 Pro+ clones.(More)
The Nedd4 family E3 ligases are key regulators of cell growth and proliferation and are often misregulated in human cancers and other diseases. The ligase activities of Nedd4 E3s are tightly controlled via auto-inhibition. However, the molecular mechanism underlying Nedd4 E3 auto-inhibition and activation is poorly understood. Here, we show that the WW(More)
When E. coli 1830/PJB4JI mating with four Citrobacter strains all were Kanamycin resistant, but a majority of KanrGens transconjugants were obtained from C-3-1. Among 3000 KanrGens 21 were auxotrophs, these are Lys-(1), Ura-(1), Arg-(2), Iso-(2), His-(2), Met-(1), Phe-(1), Tyr-(1), Ser-(1), Thr-(1), Leu-(3), Pro-(1), Ade-(3), Lac-(1), PJB4JI plasmid DNA(More)
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