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Escherichia coli W3110 was found to be 50 times more sensitive to cephamycin C than to cephalosporin C, and also markedly more sensitive to 7α-methoxycephalosporin C than to 7α-hydroxycephalosporin C. Accordingly, this organism could be used to establish a bioassay of cephalosporin C 7α-methoxylation by a Streptomyces clavuligerus cell-free extract. The(More)
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