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[16S rRNA gene sequencing for pathogen identification from clinical specimens].
By improving the extract DNA method, selecting suitable primer, developing the gene amplification and sequencing procedure, direct 16S rRNA gene sequencing can identify the pathogens from clinical specimens.
Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the screening of vanA-positive Enterococcus faecium.
- Li-jun Wang, Xin-xin Lu, W. Wu, W. Sui, Gui Zhang
- Biology, MedicineEuropean journal of mass spectrometry
- 1 December 2014
MALDI-TOF MS can be used as a screening test for discrimination between vanA-positive E. faecium andVanA-negative E. Faecium in screening vancomycin-resistant Enterococcus faecius.
[Antimicrobial resistance and virulence characteristics of Vibrio parahaemolyticus].
- Yufeng Sun, Yan-yan Fan, Yan-fei Huang, Mei Wang, Xin-xin Lu
- MedicineZhonghua yi xue za zhi
- 10 September 2013
The resistant rate of VP to ampicillin is the highest and VP has different levels of resistance against other antibiotics.
[Detection of carbapenem-producing Enterobacteriaceae by MALDI-TOF MS].
MALDI-TOF MS can detect carbapenemase-producing Enterobacteriaceae rapidly and accurately and is highly consistent with sequencing.
[Risk factors for acquired multidrug-resistant Acinetobacter baumannii colonization in respiratory intensive care unit].
Three risk factors are independently associated with the RICU-acquired colonization of MDR-AB: consciousness disturbance, use of carbapenems and mechanical ventilation.
[Correlation factor analysis of pan-drug resistant Acinetobacter baumannii strains in acquired infections].
The key control measures of acquired infections are early identification and isolation of spreader, environment and instrument disinfection, hand washing and rational uses of antibiotics.
[Comparison study between clinical diagnosis and experimental diagnosis of fungal sinusitis].
Clinical diagnosis can be used as the preliminary method for fungal sinusitis diagnosis and the demonstrative diagnosis needs to combine all above methods.
Application of gene sequencing directly to identify the pathogens in specimens.
- Xin-xin Lu, Liang Yuan, X. Wan, Jia-jing Geng
- Biology, MedicineChinese medical journal
- 1 December 2010
The use of 16S rRNA gene sequencing can improve clinical microbiology by providing better identification of unidentified bacteria or providing reference identification of unusual strains.
[Performance of locked nucleic acid probe real-time polymerase chain reaction in the detection of Aspergillus fumigatus].
- Li-jun Wang, Min Zhu, Jingjing Cao, H. Gu, Xin-xin Lu
- Biology, MedicineZhonghua yi xue za zhi
- 17 May 2011
LNA probe Real-time PCR is a promisingly accurate assay of rapidly detecting Aspergillus fumigatus practically and cost-efficiently.
Identification of bacteria and fungi by matrix-assisted laser desorption ionization-time of flight mass spectrometry
MALDI-TOF MS shows rapid turnaround time and modest reagent costs, and it will be another effective tool for microorganism diagnosis.