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OBJECTIVE To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening. DESIGN Prospective, randomized, and blinded study. SETTING Academic reproductive medicine center. PATIENT(S) Multiple euploid and aneuploid cell lines were obtained(More)
Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two unique methods of aneuploidy screening of blastomeres within the same embryo has not been conducted and may indicate whether mosaicism has been overestimated due to technical inconsistency rather than the biological phenomena. The(More)
OBJECTIVE To determine whether performing comprehensive chromosome screening (CCS) and transferring a single euploid blastocyst can result in an ongoing pregnancy rate that is equivalent to transferring two untested blastocysts while reducing the risk of multiple gestation. DESIGN Randomized, noninferiority trial. SETTING Academic center for(More)
OBJECTIVE To determine whether blastocyst biopsy and rapid quantitative real-time polymerase chain reaction (qPCR)-based comprehensive chromosome screening (CCS) improves in vitro fertilization (IVF) implantation and delivery rates. DESIGN Randomized controlled trial. SETTING Academic reproductive medicine center. PATIENT(S) Infertile couples in whom(More)
OBJECTIVE To determine both the negative and positive predictive values of comprehensive chromosome screening (CCS) results for clinical outcome. DESIGN Data obtained from two prospective, double-blinded, nonselection studies. SETTING Academic center for reproductive medicine. PATIENT(S) One hundred forty-six couples with a mean maternal age of 34.0 ±(More)
This study sought to validate a novel, minimally invasive system for embryo tracking by single nucleotide polymorphism microarray-based DNA fingerprinting of the first polar body. First polar body-based assignments of which embryos implanted and were delivered after multiple ET were 100% consistent with previously validated embryo DNA fingerprinting-based(More)
OBJECTIVE To develop and validate a quantitative real-time polymerase chain reaction (qPCR)-based method for blastocyst trophectoderm comprehensive chromosome screening (CCS) of aneuploidy. DESIGN Prospective, randomized, and blinded. SETTING Academic center for reproductive medicine. PATIENT(S) Nine cell lines were obtained from a commercial cell(More)
OBJECTIVE To prove the ability to distinguish between balanced and normal chromosomes in embryos from a translocation carrier. DESIGN Case report. SETTING Academic center for reproductive medicine. PATIENT(S) Woman with a balanced translocation causing Alagille syndrome seeking preimplantation genetic diagnosis (PGD). INTERVENTION(S) Blastocyst(More)
OBJECTIVE To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other. DESIGN Prospective, randomized, and blinded study. SETTING Academic center for reproductive medicine. PATIENT(S) Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF(More)
Methods of comprehensive microarray-based aneuploidy screening in single cells are rapidly emerging. Whole-genome amplification (WGA) remains a critical component for these methods to be successful. A number of commercially available WGA kits have been independently utilized in previous single-cell microarray studies. However, direct comparison of their(More)