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Quorum sensing (QS) is an intercellular signaling and gene regulatory mechanism, which is implicated in bacterial pathogenicity and food spoilage. Therefore, blocking bacterial QS system may prevent QScontrolled phenotypes responsible for food spoilage. In the present study, we aimed to investigate the anti-biofilm and quorum sensing inhibitory potentials(More)
Three methods were used to prepare the cell-bound exopolysaccharides (c-EPS) of Lactobacillus plantarum 70810, and the maximum yield (64.17 mg/mL) was obtained by ultrasonic extraction. After anion exchange and gel filtration chromatography, the c-EPS was fractionated as a single peak with a molecular weight of 169.6 kD. Its structural characteristics were(More)
Two released exopolysaccharides (r-EPS1 and r-EPS2) were isolated from the culture supernatant of Lactobacillus plantarum 70810 by ethanol precipitation, anion exchange and gel filtration chromatography and characterization of the structures were conducted. The r-EPS1 and r-EPS2 were homogenous polysaccharides with average molecular weights of,(More)
In the present study, the crude polysaccharides (EPS) from Lactobacillus helveticus MB2-1 were fermented with reconstituted whey medium and further fractionated on DEAE-52 cellulose and Sephadex G-100 chromatography to afford three purified fractions of EPS-1, EPS-2 and EPS-3. Then, their preliminary structures and antioxidant activities in vitro were(More)
A novel cell-bound exopolysaccharide (c-EPS) was isolated from Lactobacillus helveticus MB2-1 by ultrasonic extraction, anion exchange, and gel filtration chromatography before being structurally characterized. The c-EPS is a heteropolysaccharide with an average molecular weight of 1.83 × 10(5) Da and is composed of glucose, mannose, galactose, rhamnose,(More)
The present study aimed at investigating the potential anti-colon cancer activity of three purified exopolysaccharides fractions (LHEPS-1, LHEPS-2 and LHEPS-3) from the Lactobacillus helveticus MB2-1. The experimental evidence showed that LHEPS-1 significantly inhibited cell proliferation of human colon cancer Caco-2 cells in both time- and(More)
In the present study, a released exopolysaccharide (r-EPS1) from L. plantarum 70810 was modified by acetylation, phosphorylation and carboxymethylation. Scanning electron micrograph (SEM) and thermogravimetric analysis (TGA) showed the r-EPS1 derivatives had different surface morphology and thermal behavior. Compared with r-EPS1, the derivatives exhibited(More)
The K-means algorithm based on partition and the DBSCAN algorithm based on density are analyzed. Combining advantages with disadvantages of the two algorithms, the improved algorithm DBSK is proposed. Because of the partition of data set, DBSK reduces the requirement of memory; the method of computing variable value is put forward; to the uneven data set,(More)
BACKGROUND Prostate cancer is the most common cancer in men in the United States. Fucoidan is a bioactive polysaccharide extracted mainly from algae. The aim of this study was to investigate anti-tumor and anti-angiogenic effects of fucoidan in both cell-based assays and mouse xenograft model, as well as to clarify possible role of JAK-STAT3 pathway in the(More)
We have developed a novel bio-tofu, made from mixed soy and cow milk (MSCM), using Lactobacillus helveticus MB2-1 and Lactobacillus plantarum B1-6 incorporated with microbial transglutaminase (MTGase) as coagulant. MTGase was added to improve the textural properties and suit for cooking. However, the effect of MTGase on the digestion of mixed-protein(More)