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A method of high performance liquid chromatography-fluorescence detection (HPLC-FLD) for the simultaneous determination of kynurenine (Kyn) and kynurenic acid (KYNA) in serum was developed. A 20 microL of the supernatant of a serum sample deproteinized by 5% perchloric acid solution was separated on a Hypersil C8 column (300 mm x 6.0 mm, 10 microm) with an(More)
We investigated the activation and pathophysiological roles of indoleamine 2,3-dioxygenase (IDO) and kynurenine aminotransferase (KAT) in patients with ischemic stroke. Patients were recruited from the acute stroke unit of a general hospital within 24 hours post-stroke. The immune transmission turbidity method was used to determine the concentration of(More)
Nineteen cases of acute accidental laser injury of the human retina and two groups of laser workers with chronic retinal damage were reviewed. Most acute cases had macular injury and vision impairment; the chronic cases usually suffered from nonspecific eye complaints. Pathological and photochemical studies of laser injury to rabbit retina were also made(More)
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