Learn More
The scandal of melamine-adulterated infant formula in China in September 2008 demanded the need to assess the extent of melamine contamination in the environment and food products and possible risks of consuming melamine-contaminated diets. In this work, our extensive work tested water, soil and crop samples from 21 provinces in China. Soils nearby and(More)
Many countries have introduced maximum residue limits for melamine in foods since its adulteration in infant formula in China. However, more animal feeding studies are needed to understand the fate of melamine-contaminated animal feed. In this study, the melamine contents in tissues and serum were tested using LC-MS/MS for up to 60 days for lambs fed with(More)
A specific mechanism whereby inflammation may contribute to cardiovascular diseases (CVD), insulin resistance (IR) and type II diabetes is the induction of endothelial dysfunction placing the vascular endothelium in a key unifying position for the shared pathogenesis of these diseases. However, the mechanisms by which PCBs induce endothelial cell(More)
Microcystins (MCs) are a family of cyclic heptapeptides that are produced by blooming algae Microcystis. MCs have been implicated in the development of liver cancer, necrosis and even intrahepatic bleeding. Effective prophylactic approaches and complete removal of MCs are urgently needed. Accumulating evidence suggests that microcystin-LR (MC-LR)-induced(More)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs) are persistent organic pollutants found as complex mixtures in the environment throughout the world. Therefore, humans are ubiquitously and simultaneously exposed to TCDD and PCBs. TCDD and PCBs alone have been linked to atherosclerosis. However, the effects of interactions or(More)
OBJECTIVE In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells, the effects of PPARγ and POX-induced ROS were explored. METHODS [3- (4, 5)-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, Annexin V and PI staining using FACS, plasmid transfection, ROS formation detected by DCFH staining, RNA(More)
Both of ceramide and PPARgamma ligand can trigger cancer cell apoptosis. We here show that C2-ceramide can modulate PPARgamma expression level and its transcriptional activity and results in apoptosis in HT29 cells. Administration of PPARgamma specific antagonist GW9662 partially prevents HT29 cells from apoptosis. Furthermore, MAP kinase pathway provided a(More)
Both of ceramide and PPARγ ligand can trigger cancer cell apoptosis. We here show that C2-ceramide can modulate PPARγ expression level and its transcriptional activity and results in apoptosis in HT29 cells. Administration of PPARγ specific antagonist GW9662 partially prevents HT29 cells from apoptosis. Furthermore, MAP kinase pathway provided a potential(More)
The application of biotechnological products in the feed industry has undergone explosive growth in recent years, and phytase from microorganism accounts for one-third of the entire feed enzyme market. In this study, some differences in the composition of protein and denaturation temperature between two commercial phytases were determined by HPLC and(More)
Standard methods for determining the raw material content of compound feed are little exploited, except for the identification of meat and bone meal in feeds. In this work, near-infrared (NIR) spectroscopy and real-time polymerase chain reaction (PCR) were applied in order to establish new and fast methods for quantification of soybean meal content in(More)