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Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of mmu-mir-143 (miR-143) in mammalian ovary development have not been studied in vitro. We investigated the expression and function of miR-143 in the mouse ovary during primordial follicle formation. Real-time polymerase chain reaction analysis showed that miR-143(More)
The interaction of chronic alcohol consumption with estrogen has been recognized in various tissues; however, the potential mechanism has not been clearly defined. In this study, we made the following observations: (a) 0.01% (v/v) ethanol (corresponding to 1.7 mM) significantly elevated estrogen receptor α (ERα) protein content, stimulated activator(More)
Herein, we propose a new content based image retrieval method. The novelty of our approach lies in the applied image similarity measure : Unlike traditional features like color, texture or shape, our measure is based on a painted representation of the original image. We use paintbrush stroke parameters as features. These strokes are produced by a stochastic(More)
A novel strategy for the preparation of in-column adenosine deaminase (ADA) microreactor and rapid screening of enzyme inhibitors in natural extracts was demonstrated. In this approach, ADA was encapsulated in anionic polyelectrolyte alginate that was immobilized on the surface of fused-silica capillary via ionic binding technique with cationic(More)
A facile protocol to prepare highly effective and durable in-line enzyme bioreactors inside capillary electrophoresis (CE) columns was developed. To demonstrate the methodology, l-glutamic dehydrogenase (GLDH) was selected as the model enzyme. GLDH was first immobilized onto 38-nm-diameter gold nanoparticles (GNPs), and the functionalized GNPs were then(More)
Ultrasonically assisted extraction and Soxhlet extraction using 95% ethanol were performed to obtain ultrasonically assisted extract of Agrimonia pilosa (UEA) and Soxhlet extract of Agrimonia pilosa (SEA). The effects of the different extracting methods on the total phenolic (TP) and total flavonoid (TF) content of the extracts from Agrimonia pilosa were(More)
In this paper, we will show how non-photorealistic rendering (NPR) can take a new role in content-based image retrieval (CBIR). We propose a content-based image retrieval method. The novelty is that it is based on the painting representation of images, obtained by Stochastic Paintbrush Transformation (SPT), which automatically simulates a painting process.(More)
Several ecological restoration projects have been constructed along urban rivers in North China to purify reclaimed municipal wastewater and improve the water quality of urban rivers. These projects attempt to address several environmental issues, including treating water contamination that is not fully remediated through standard wastewater treatment. This(More)
The establishment of the primordial follicle pool is crucial for fertility in mammalian females, and the interruption of overall micro-RNA production byDicer1conditional knockout in the female reproductive system results in infertility. However, there are few reports about the functions of individual micro-RNA in regulating primordial follicle assembly. The(More)