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The nonpolymorphic soluble HLA-G1 (sHLA-G1) isoform has been reported to be secreted by trophoblast cells at the materno-fetal interface, suggesting that it may act as immunomodulator during pregnancy. In this paper, we report that affinity-purified beta2-microglobulin-associated sHLA-G1 triggered apoptosis in activated, but not resting CD8+ peripheral(More)
Different HLA-G monoclonal antibodies (mAbs) were first evaluated for their capability to identify soluble HLA-G (sHLA-G) in ELISA. Three of them, namely 87G, BFL.1 and MEM-G/9, when used as coating mAbs together with W6/32 capture mAb, identified beta2-microglobulin (beta2m)-associated-sHLA-G but not soluble HLA-B7 (sHLA-B7) in cell culture supernatants(More)
To establish a method for determination of cantharidin content in Mylabris phalerata. Pallas, and to study its inhibitory and apoptosis-inducing effects on human hepatoma cell line HepG2. Agilent column (TC-C18, 250 mm × 4.6 mm, 5 μm); mobile phase: methanol-water (volume ratio: 20:80); flow rate: 1.0 ml/min; UV detection wavelength: 230 nm; and column(More)
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