Xiaoliang He

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High soil salinity is a major abiotic stress in plant agriculture worldwide. Here, we report the characterization of a novel aquaporin gene TaNIP (Triticum asetivum L. nodulin 26-like intrinsic protein), which was involved in salt tolerance pathways in plants. TaNIP was identified and cloned through the gene chip expression analysis of a salt-tolerant wheat(More)
Wheat vacuolar H+-ATPases (V-ATPase) subunit B, named TaVB, was isolated from the salt-tolerant wheat RH8706-49 and used to transform Arabidopsis plants. TaVB-expressed Arabidopsis has a higher germination rate, root length, V–H+-ATPase activity, and overall salt tolerance than the wild type, indicating that expression of the gene can affect salt tolerance(More)
A microarray analysis of the salt-resistant wheat mutant, RH8706-49, revealed a salt-induced gene containing a conserved DUF581 domain. The gene was cloned and designated as Triticum aestivum salt-related hypothetical protein (TaSRHP) and submitted to GenBank (accession no. GQ476575). Over-expression of TaSRHP in wild-type Arabidopsis thaliana cv. Columbia(More)
TaGSK1, a salt-inducible gene, has a very high identity with mammalian GSK3 which is a highly conserved serine/threonine protein kinase that regulates glycogen synthesis. Overexpression of TaGSK1 reduces cellular osmotic turgor and cellular Na+ content and enhances salt tolerance of transgenic Arabidopsis plants. Biochemical analysis of transgenic lines of(More)
Mononuclear cell (MNC) infiltrate is one of the earliest pathological changes in systemic sclerosis (SSc) skin. However, little is known about the recruitment of these cells into skin lesions. Recently, the role of chemokines has been suggested in the pathogenesis of SSc. Here we studied the expressions and distributions of CC chemokine CCL20 and its(More)
Genes for V-H(+)-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants. Overexpression of these genes in Arabidopsis thaliana improved its salt tolerance, and increased the activities of V-H(+)-ATPase and Na(+)/H(+) exchange, with the largest increase in plants(More)
In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking. In addition, ATDC-5 was inoculated into the scaffolds incorporating the chitosan(More)
An unknown d-tagatose 3-epimerase (DTE) containing a IoIE domain was identified and cloned from Escherichia coli. This gene was subcloned into the prokaryotic expression vector pET-15b, and induced by IPTG in E. coli BL21 expression system. Through His-select gel column purification and fast-protein liquid chromatography, highly purified and stable DTE(More)
Bacteria predominantly adapt to environmental changes to ensure their growth and proliferation through one-component, two-component and three-component regulatory systems. Conjugative pilus expression (Cpx) system is one of the two-component regulatory systems in gram-negative bacteria. It is composed of the membrane-anchored sensor kinase CpxA, the(More)
Ethylene-responsive factors (ERFs) are commonly considered to play an important role in pathogen defense responses. However, only few of ERF members have been characterized in Sea island cotton (Gossypium barbadense). Here, we reported a novel AP2/ERF transcription factors gene, named GbERFb which was cloned and identified from Sea island cotton by RACE.(More)