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Purification and characterization of extracellular amylase from the marine yeast Aureobasidium pullulans N13d and its raw potato starch digestion
Abstract The extracellular glucoamylase in the supernatant of the cell culture of the marine yeast Aureobasidium pullulans N13d was purified to homogeneity with a 7.3-fold increase in specificExpand
Biofabrication of biosilica-glass by living organisms.
The present stage of knowledge on structure, biochemical composition, and mechanisms of biosilica formation is reported, focusing particularly on sponges because of the enormous (nano)biotechnological potential of the enzymes involved in this process. Expand
Bioorganic/inorganic hybrid composition of sponge spicules: matrix of the giant spicules and of the comitalia of the deep sea hexactinellid Monorhaphis.
The complex bioorganic/inorganic hybrid composition and structure of the Monorhaphis spicules might provide the blueprint for the synthesis of bio-inspired material, with unusual mechanical properties (strength, stiffness) without losing the exceptional properties of optical transmission. Expand
Apposition of silica lamellae during growth of spicules in the demosponge Suberites domuncula: biological/biochemical studies and chemical/biomimetical confirmation.
It is shown that the appositional growth of the spicules in radial and longitudinal direction proceeds in the extracellular space along hollow cylinders; their surfaces are formed by silicatein. Expand
Formation of giant spicules in the deep-sea hexactinellid Monorhaphis chuni (Schulze 1904): electron-microscopic and biochemical studies
It is suggested that, in the Hexactinellida, the growth of the spicules is mediated by silicatein or by a silicatesin-related protein, with the orientation of biosilica deposition being controlled by lectin and collagen. Expand
Isolation of the silicatein-α interactor silintaphin-2 by a novel solid-phase pull-down assay.
Findings indicate that silintaphin-2 might mediate signal transduction during spiculogenesis or may play a more direct role during biosilica formation, in concert with silicatein. Expand
Evagination of Cells Controls Bio-Silica Formation and Maturation during Spicule Formation in Sponges
The enzymatic-silicatein mediated formation of the skeletal elements, the spicules of siliceous sponges starts intracellularly and is completed extracellularly, and the sequence of events that govern spicule formation is termed bio-inorganic self-organization. Expand
The role of biosilica in the osteoprotegerin/RANKL ratio in human osteoblast-like cells.
Biosilica stimulated OPG synthesis in osteoblast-like cells counteracts those pathways that control RANKL expression and function (e.g. maturation of pre-osteoclasts and activation of osteoclasts). Expand
Sponge spicules as blueprints for the biofabrication of inorganic–organic composites and biomaterials
First bioinspired approaches implement recombinant silicatein and silintaphin-1 for applications in the field of biomedicine (biosilica-mediated regeneration of tooth and bone defects) or micro-optics (in vitro synthesis of light waveguides) with promising results. Expand
Bio-silica and bio-polyphosphate: applications in biomedicine (bone formation).
Since bio-silica and bio-polyP also induce the expression of the key mediator BMP2 which directs the differentiation of bone-forming progenitor cells to mature osteoblasts and in parallel inhibits the function of osteoclasts, they are promising candidates for treatment of osteoporosis. Expand