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sgRNA:Cas9 at the YSA gene sgRNA2 site in rice. Alleles shown were amplified from genomic DNA isolated from 6 independent T0 transgenic plants separately and sequenced after cloned into vectors. The wild type sequence is shown at the top with the PAM sequence highlighted in magenta and the target sequence in cyan. Red dashes, deleted bases; red bases,(More)
The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has emerged as a powerful tool for targeted gene editing in many organisms, including plants. However, all of the reported studies in plants focused on either transient systems or the first generation after the CRISPR/Cas system was stably transformed into(More)
The problems of relaxed quadratic stability conditions, fuzzy observer designs and H∞ controller designs for T-S fuzzy systems have been studied. First new stability conditions are obtained by relaxing the stability conditions derived in previous papers. Secondly, new fuzzy observers based on the relaxed stability conditions for the T-S fuzzy systems have(More)
Methods of deep neural networks (DNNs) have recently demonstrated superior performance on a number of natural language processing tasks. However, in most previous work, the models are learned based on either unsupervised objectives, which does not directly optimize the desired task, or singletask supervised objectives, which often suffer from insufficient(More)
This paper considers the speci…cation and estimation of social interaction models with network structures and the presence of endogenous, contextual and correlated e¤ects. With macro group settings, group-speci…c …xed e¤ects are also incorporated in the model. The network structure provides information on the identi…cation of the various interaction e¤ects.(More)
A 12 bit 2.9 GS/s current-steering DAC implemented in 65 nm CMOS is presented, with an dBc beyond 1 GHz while driving a 50 load with an output swing of 2.5 and dissipating a power of 188 mW. The SFDR measured at 2.9 GS/s is better than 60 dB beyond 340 MHz while the SFDR measured at 1.6 GS/s is better than 60 dB beyond 440 MHz. The increase in performance(More)
In vitro drug metabolism studies, which are inexpensive and readily carried out, serve as an adequate screening mechanism to characterize drug metabolites, elucidate their pathways, and make suggestions for further in vivo testing. This publication is a sequel to part I in a series and aims at providing a general framework to guide designs and protocols of(More)
The transition from dormancy to germination in seeds is a key physiological process during the lifecycle of plants. Abscisic acid (ABA) is the sole plant hormone known to maintain seed dormancy; it acts through a gene expression network involving the transcription factor ABSCISIC ACID INSENSITIVE 3 (ABI3). However, whether other phytohormone pathways(More)