Xiao-Yu Gao

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Osteoblasts can synthesize the insulin-like growth factors (IGFs) and the IGF-binding proteins (IGFBPs), which may either enhance or attenuate IGF-stimulated bone cell proliferation. Since estrogen induced osteoblastic differentiation and proliferation through an estrogen-responsive gene in target cells, we investigated the effects of estrogen on IGFBP-6(More)
Although thymosin beta 4 (Tβ4) is known to play a role in hair growth, its mechanism of action is unclear. We examined the levels of key genes in a Tβ4 epidermal-specific over-expressing mouse model and Tβ4 global knockout mouse model to explore how Tβ4 affects hair growth. By depilation and histological examination of the skin, we confirmed the effect of(More)
OBJECTIVE To evaluate the effects of retinoic acid (RA) on expression of bone morphogenetic protein 7 (BMP-7) in rat fetus with cleft palate, and the effects of RA on proliferation and apoptosis of osteoblasts. METHODS All-trans RA (ATRA) was used to induce congenital cleft palate in Wistar rat BMP-7 mRNA expression in maxillary bone tissue of fetal rats(More)
It has been reported that protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D) plays an important role in cancer tumorigenesis. However, the clinical and functional significance of PPM1D expression has not been characterized previously in non-small cell lung cancer (NSCLC). The purpose of this study was to assess PPM1D expression and to explore its(More)
OBJECTIVE To investigate the relationship between the HLA-DQB1 allele polymorphisms and the clinical features of 15 familial myasthenia gravis (MG) cases in north China. METHODS By polymerase chain reaction-sequence specific primers (PCR-SSP), the HLA-DQB1 gene polymorphisms were determined in 64 MG patients (15 familial and 49 sporadic) and 52 healthy(More)
OBJECTIVE To investigate the effect of the environmental carcinogenic factor-TCDD (2, 3, 7, 8-tetrachlorodibenzo-p-dioxin) on cell apoptosis and gene regulation of insulin-like growth factor binding protein 6 (IGFBP-6) in osteogenic sarcoma (SaOS-2) cells. METHODS The SaOS-2 cells were cultured with TCDD (1 x 10(-9), 1 x 10(-8), 1 x 10(-7) mol/L) for 24(More)
在超积累植物东南景天对镉的区隔化过程中, 叶肉细胞等内含大型液泡的薄壁细胞起重要作用。本文旨在建立并优化其叶肉细胞原生质体和液泡的提取和纯化技术, 在技术层面上为东南景天的镉区隔化机理研究奠定基础, 有助于深入探明其超积累镉的生理与分子机理。 优化了东南景天叶片原生质体的提取和纯化技术, 并建立了能较高效率获得膜完整性好、数量多、纯度高的液泡提取方法。 主要包括原生质体提取、液泡粗提和液泡纯化。原生质体提取: 取东南景天叶片, 切成1~2 mm的细条状后浸入经预热过的细胞裂解液中, 震荡2 h 后过滤, 离心清洗后获得原生质体。液泡粗提: 采用1-丙磺酸浓度为0.675 mmol/L 的原生质体裂解液裂解原生质体, 离心后获得粗提的液泡, 并加入含0.8 mol/L 甘露醇的液泡保护液。液泡纯化:(More)
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