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The higher ranks of the class Actinobacteria were proposed and described in 1997. At each rank, the taxa were delineated from each other solely on the basis of 16S rRNA gene sequence phylogenetic clustering and taxon-specific 16S rRNA signature nucleotides. In the past 10 years, many novel members have been assigned to this class while, at the same time,(More)
Strains YIM 90625(T), YIM 90630(T), YIM 90636(T) and YIM 90694(T) were isolated from a salt lake in Xinjiang province, north-west China, and were subjected to a polyphasic analysis to determine their taxonomic positions. All isolates were moderately halophilic and were able to grow at NaCl concentrations up to 15 or 20 % (w/v). The genomic DNA G+C contents(More)
A novel halophilic, filamentous, actinomycete strain, designated YIM 90648(T), was isolated from a salt lake in Xinjiang Province, north-west China, and subjected to a polyphasic taxonomic study. Optimal growth occurred at 37 degrees C, pH 7.0-8.0 and 15% (w/v) NaCl. The aerial mycelium of strain YIM 90648(T) formed long chains of spores at maturity and the(More)
During the course of screening for industrially important microorganisms, an alkali-tolerant and thermotolerant actinomycete, strain DAS 131T, was isolated from a soil sample collected from the Gulbarga region, Karnataka province, India. The strain was characterized by a polyphasic approach that showed that it belonged to the genus Streptomyces. Growth was(More)
The actinomycete strain YIM 90462T was isolated from a salt mine sample collected from Yunnan province, south-west China. Comparative 16S rRNA gene sequence analysis demonstrated that the isolate, which had sequence similarities of more than 97 % to Thermobifida alba, Thermobifida fusca and Thermobifida cellulosilytica, was a candidate member of the genus(More)
An actinomycete strain, designated YIM 91355(T), was isolated from a soil sample collected from a hypersaline soil in Xinjiang Province, China, and its taxonomic position was investigated by using a polyphasic approach. The strain grew well on most media tested and no diffusible pigment was produced. The aerial mycelium of this organism was well developed(More)
A Gram-staining-positive, facultatively anaerobic, non-motile and moderately halophilic actinobacterium, designated YIM 93306(T), was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic study. Strain YIM 93306(T) grew in the presence of 2-16 % (w/v) NaCl and did not grow without NaCl. The peptidoglycan(More)
Thousands of hot springs are located in the north-eastern part of the Yunnan-Tibet geothermal zone, which is one of the most active geothermal areas in the world. However, a comprehensive and detailed understanding of microbial diversity in these hot springs is still lacking. In this study, bacterial and archaeal diversities were investigated in 16 hot(More)
Diversity of Crenarchaeota was investigated in eight terrestrial hot springs (pH 2.8-7.7; temperature 44-96 degrees C) located in Tengchong, China, using 16S rRNA gene phylogenetic analysis. A total of 826 crenarchaeotal clones were sequenced and a total of 47 operational taxonomic units (OTUs) were identified. Most (93%) of the identified OTUs were closely(More)
The taxonomic position of an actinomycete, designated SCSIO 00231(T), isolated from a sediment sample collected from the northern South China Sea, was determined by using a polyphasic approach. The organism formed fragmented substrate hyphae and sparse aerial mycelium on modified ISP 2 medium. Phylogenetic analysis based on the 16S rRNA gene sequence showed(More)