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We have conducted alleletyping of two novel cell lines derived from glioblastoma multiforme, which appear to have arisen from different glial lineages, by using 76 fluorescently labeled oligonucleotide primers amplifying microsatellite loci covering the entire human genome. One cell line, Hu-O-2A/Gb1, expresses antigens and metabolic profiles characteristic(More)
We have examined the genetic aberrations in two near-diploid glioblastoma multiforme cell lines that appear to have arisen from different glial lineages. One cell line, Hu-O-2A/Gb1, expresses antigens and metabolic profiles characteristic of the oligodendrocyte-type-2 astrocyte (0-2A) lineage of the rat central nervous system. This line generates, in vitro,(More)
The use of lactose as inducer for the expression of dextransucrase was investigated in high density of Escherichia coli. Different culture conditions including carbon source, nitrogen sources, inorganic salts, inoculum density (OD 600) and lactose concentrations were optimized, and high-level expression of dextransucrase in E. coli was achieved via an(More)
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