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BACKGROUND Sirtuin 3 (SIRT3) is one of the seven mammalian sirtuins, which are homologs of the yeast Sir2 gene. SIRT3 is the only sirtuin with a reported association with the human life span. Peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) plays important roles in adaptive thermogenesis, gluconeogenesis, mitochondrial(More)
Knowledge of the polyprotein cleavage sites by HIV protease will refine our understanding of its specificity, and the information thus acquired is useful for designing specific and efficient HIV protease inhibitors. The pace in searching for the proper inhibitors of HIV protease will be greatly expedited if one can find an accurate, robust, and rapid method(More)
Support Vector Machine (SVM), which is one class of learning machines, was applied to predict the subcellular location of proteins by incorporating the quasi-sequence-order effect (Chou [2000] Biochem. Biophys. Res. Commun. 278:477-483). In this study, the proteins are classified into the following 12 groups: (1) chloroplast, (2) cytoplasm, (3)(More)
The support vector machines (SVMs) method was introduced for predicting the structural class of protein domains. The results obtained through the self-consistency test, jack-knife test, and independent dataset test have indicated that the current method and the elegant component-coupled algorithm developed by Chou and co-workers, if effectively complemented(More)
In this paper, we apply a new machine learning method which is called support vector machine to approach the prediction of protein structural class. The support vector machine method is performed based on the database derived from SCOP which is based upon domains of known structure and the evolutionary relationships and the principles that govern their 3D(More)
A cDNA clone (designated as SsPR10, GenBank Accession Number AY660753 ) encoding a PR10 protein from yellow-fruit nightshade (Solanum surattense) was isolated and characterized. SsPR10 encoded a 160-amino-acid polypeptide with a predicted molecular mass of 17.58 kDa and pI of 5.29. Sequence alignments showed that SsPR10 had high identity (68.1%) with(More)
A new dehydrin ERD10 gene was cloned and characterized from Brassica napus (designated as Bndhn ERD10). The full-length cDNA of Bndhn ERD10 was 1114 bp and contained an open reading frame of 816 bp encoding a protein of 271 amino acid residues. The deduced Bndhn ERD10 protein contained an 8-serine residue domain and two conserved repeats of the(More)
Dysregulated miRNAs in gastric cancer are usually screened by miRNA microarray from clinical samples, however, reports have indicated that results of each miRNA microarray screening are considerably different, and dysregulated miRNAs, especially downregulated miRNAs were contradictory. In view of this, the Human Cancer Pathway Finder miRNA PCR array was(More)
GLUT4 is the main glucose transporter activated by insulin in skeletal muscle cells and adipocytes. GLUT4 storage vesicles (GSVs) traffic in endocytic and exocytic compartments. In the basal state, GLUT4 compartments are preferentially sequestered in perinuclear deposits wherein stimuli including insulin and non-insulin factors can increase GLUT4 vesicle(More)
In the present study, the antidepressant-like effect of bacopaside I, a saponin compound present in the Bacopa monniera plant, was evaluated by behavioral and neurochemical methods. Bacopaside I (50, 15 and 5 mg/kg) was given to mice via oral gavage for 7 successive days. The treatment significantly decreased the immobility time in mouse models of despair(More)