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Escherichia coli K-12 carries a gene for a protein denoted ClyA or SheA that can mediate a cytolytic phenotype. The ClyA protein is not expressed at detectable levels in most strains of E. coli, but overproduction suitable for purification was accomplished by cloning the structural gene in an hns mutant strain. Highly purified ClyA protein was cytotoxic to(More)
The murine macrophage-like cell line J774.A1 ingests and allows intracellular growth of Francisella tularensis. We demonstrate that, after 24 h of infection, a pronounced cytopathogenicity resulted and the J774 cells were undergoing apoptosis. Despite this host cell apoptosis, no decrease in bacterial numbers was observed. When internalization of bacteria(More)
Global Positioning System (GPS) measurements in China indicate that crustal shortening accommodates most of India's penetration into Eurasia. Deformation within the Tibetan Plateau and its margins, the Himalaya, the Altyn Tagh, and the Qilian Shan, absorbs more than 90% of the relative motion between the Indian and Eurasian plates. Internal shortening of(More)
The TCR repertoire of an epitope-specific CD8(+) T cell population remains poorly characterized. To determine the breadth of the TCR repertoire of a CD8(+) T cell population that recognizes a dominant epitope of the AIDS virus, the CD8(+) T cells recognizing the tetrameric Mamu-A*01/p11C(,CM) complex were isolated from simian immunodeficiency virus(More)
Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates a hemolytic phenotype. We show here that highly purified ClyA and ClyA-expressing E. coli were cytotoxic and apoptogenic to fresh as well as cultured human and murine monocytes/macrophages.
Both therapies of traditional crude herb moxibustion and drug acupoint application were used in 209 cases of allergic asthma to compare their long-term and short-term therapeutic effects and to analyze the relationship between clinic therapeutic effects of both therapies and differential types of the disease. The results showed that the short-term total(More)
The purpose of this study was to evaluate the relationship between injury to endothelial cells and the plasma endothelin level after gunshot wounds. Forty dogs were divided into four groups. The dogs in group 1 served as a control. The dogs in groups 2,3, and 4, respectively, were shot in both hind legs, in the hypogastric part of the abdomen, or in the(More)
The active constituents of Herba Epimedii can raise the hypothalamic monoamine neurotransmitters levels in aging male rats and improve the learning and memory in aging rats and mice. It can also inhibit the activities of AchE both in brain tissues and whole blood in mice.
By subcutaneous inoculation of 10(8) CFU of enterohemorrhagic E. coli O157:H7, specific-pathogen-free mice revealed most of the symptoms and histological changes observed in patients. The histological changes in intestine were mainly seen in the distal parts of small intestine and the cecum. Vacuolation of villi in the cecum was also observed. The(More)
Herba Epimedii is a traditional Yang invigorating Chinese herb widely used in clinic. The experimental results have shown that ESPS obviously increases superoxide dismutase(SOD) activity of red cells and liver in aged mice and rats, and increases glutathione peroxidase(GSH-Px) activity of red cells in aged mice. On the other hand, ESPS obviously helps to(More)