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Transgenesis has been used for expressing human lysozyme (hLZ) in the milk of livestock to improve their disease resistance. Here we describe a human lactoferrin (hLF) BAC as a candidate vector for high-level expression of hLZ in the milk of transgenic mice. Using recombineering, hLF genomic DNA in the hLF BAC was replaced by the hLZ gene (from the ATG(More)
This paper studies on knowledge retrieval technology in product conceptual design. In this study, a two-layer retrieval technology is proposed. One is to get the similarities of function units through matching function attribute values which is weighted. The concepts of similarity vector are proposed for searching similar function units or module. Another,(More)
In this work, the properties of dopamine and dopamine-quinone on gold nanoparticles (Au NPs) surface were studied by the constructed Au NPs/dopamine sensing platform using surface enhanced Raman scattering (SERS) spectroscopy. Interestingly, the Au NPs/dopamine-quinone exhibited the characteristic Raman band at 1270, 1335 and 1480 cm(-1) at pH 10.0,(More)
New types of porphyrin nano- and micro-particles composed of J- and H-heteroaggregates were prepared by electrostatic self-assembly of two oppositely charged porphyrins, tetrakis(4-trimethylammoniophenyl)porphyrin (H2TAPP4+) and tetrakis(4-sulfonatophenyl)porphyrin cobalt(II) (CoTPPS4-), in aqueous solutions. Transmission electron microscopy (TEM) images(More)
The redox properties of cytochrome c (Cyt c), hemoglobin (Hb) and myoglobin (Mb) were studied based on electrostatic interactions between Thioglycolic acid (TGA) capped CdSe/ZnS quantum dots (QDs) and proteins. Results indicated that only Cyt c quenched the fluorescence of the QDs at pH>8.0. Under the optimized conditions, a significant fluorescence(More)
A novel and facile approach for preparing highly oriented porphyrin metal-organic framework films on various substrates is explored by employing self-directed assembly of silanized porphyrin cobalt monomers (SPCMs). The three-dimensional ordered porphyrin films display excellent photo-electronic performance.
Knowledge can be acquired from organization inside and exterior. The knowledge inside organization is limited and the organization external knowledge is important to advance organization competition ability. Knowledge acquisition out of organization plays an extremely important role in organization knowledge management. This study proposed a knowledge(More)
Neutron emission measurement at the HL-2A tokamak device with a liquid scintillation detector is described. The detector was placed at a location with little structure material in the field of view, and equipped with a gain monitoring system which could provide the possibility to evaluate the gain variation as well as to correct for the detector response.(More)