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The 7 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) gene from Escherichia coli HB101 was cloned and expressed in E. coli DH1. The hybrid plasmid pSD1, with a 2.8-kbp insert of chromosomal DNA at the BamHI site of pBR322, was subcloned into pUC19 to construct plasmid pSD3. The entire nucleotide sequence of an inserted PstI-BamHI fragment of plasmid pSD3(More)
Protease II gene of Escherichia coli HB101 was cloned and expressed in E. coli JM83. The transformant harboring a hybrid plasmid, pPROII-12, with a 2.4 kbp fragment showed 90-fold higher enzyme activity than the host. The whole nucleotide sequence of the inserted fragment of plasmid pPROII-12 was clarified by the dideoxy chain-terminating method. The(More)
Cells possess within their epigenetic repertoire the ability to undergo an active process of cellular suicide termed programmed (or apoptotic) cell death. This programmed cell death process involves an epigenetic reprogramming of the cell that results in an energy-dependent cascade of biochemical and morphologic changes (also termed apoptosis) within the(More)
BACKGROUND More than 95% of metastatic androgen independent prostatic cancer cells per day are in a proliferatively quiescent G0 state [Berges et al.: Clin Cancer Res 1:473-480, 1995] limiting their responsiveness to anti-proliferative chemotherapeutic agents. Novel therapeutics capable of activating the programmed (apoptotic) death pathway in these cells(More)
Proliferating cells characteristically undergo programmed (i.e. apoptotic) death if their progression through the cell cycle is sufficiently perturbed. To determine whether androgen ablation-induced programmed death of prostatic glandular cells involves apoptosis triggered by recruitment of nonproliferating cells into a perturbed cell cycle, rat ventral(More)
BACKGROUND Caspases are a family of cysteine proteases capable of characteristically cleaving after an aspartic acid residue. Various members of the caspase family (e.g., caspases 8 and 9) have been implicated as critical initiators in the signaling phase, while others (e.g., caspases 3, 6, and 7) have been implicated in the effector or execution phase of(More)
The availability offered by current data replication and update algorithms varies with dynamically changing conditions which include the network configuration and system load. With dynamic adaptability, systems can switch to an appropriate mechanism to improve perFormance and availability, In this paper, we present an algorithm to estimate the overall(More)
Multi-media messages are typically very large and vary from tens to thousands of kilobytcs in size. This paper investigates the transmission of large multi-media messages on an Ethernet LAN, Internet WAN, and an ATM LAN. We proposed a UDP cooling method by introducing a. short delay between each datagram for large message transmissions. Experiments show(More)
The genetic pathway for the activation and completion of programmed death of cells is as complex and well regulated as the pathway for cell proliferation. The identification of both the genetic elements in the signal transduction pathway involved with the initiation of programmed cell death, as well as the cellular machinery involved with DNA and cellular(More)
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