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Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli
The gene deletions critical to reducing acetate accumulation during aerobic growth and citramalate production in metabolically engineered E. coli strains are identified, significantly improving a potential process for the production of this five-carbon chemical.
Production of citramalate by metabolically engineered Escherichia coli
Citramalic acid (citramalate) is a five carbon hydroxy‐dicarboxylic acid and potential precursor for the production of methacrylic acid from renewable resources. We examined citramalate production in
Effects of additives on sucrose-derived activated carbon microspheres synthesized by hydrothermal carbonization
In the hydrothermal carbonization of carbohydrates, such as sucrose as raw material in this study, activated carbon microspheres were synthesized by two steps of hydrothermal carbonization (180 °C)
Wet oxidation and absorption procedure for NOx removal
Engineered citrate synthase improves citramalic acid generation in Escherichia coli.
Results demonstrate protein engineering can be used as an effective tool to redirect carbon flux by reducing enzyme activity and improve the microbial production of traditional commodity chemicals.
Adaptation of Escherichia coli to Elevated Sodium Concentrations Increases Cation Tolerance and Enables Greater Lactic Acid Production
Pyruvate formate lyase knockouts were constructed in strains MG1655 and ALS1187 to determine whether increased Na+ tolerance afforded increased anaerobic generation of lactate, and showed significant differences in fed-batch fermentations.
Synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli
These studies revealed that gltA coding citrate synthase, leuC coding 3-isopropylmalate dehydratase, and acetate pathway genes play important roles in elevating citramalate and minimizing acetate formation.
Effect of overexpressing nhaA and nhaR on sodium tolerance and lactate production in Escherichia coli
Although overexpressing the genes did not improve growth rate at high Na+ concentrations, the overexpression of nhaA and nhaR together led to a 25% increase in lactate production.
Water-Based UV-Curable Polyurethane Based on Wheat Straw Lignin Obtained by Ethanol Extraction
The aim of this paper is to discuss the application of ethanol-dissolved lignin in preparing high value products. Lignin was extracted from wheat straw by ethanol and used to prepare water-based